Abstract
The sucrose-negative strains ofYersinia enterocolitica biotype 3 serotype 03 phage type 2 were isolated from cecal contents and oral cavity swabs of slaughtered pigs and from a swab of a skinner at the slaughterhouse. These organisms differed fromY. kristensenii, determined by assaying the antibiotic susceptibilities to ampicillin, carbenicillin, and cephalothin. These organisms showed positive reactions in the presence of 44 Md plasmid, a calcium dependency, autoagglutination activity, and produced diarrhea in mice and a negative reaction for pyrazinamidase activity. Plasmid digestion with restriction endonucleases isolated from this organism showed the same patterns in biotypes 3 and 4 serotype 03. Therefore, the sucrose-negative strains ofY. enterocolitica biotype 3 serotype 03 are apparently pathogenic.
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Literature Cited
Baker PM, Farmer III JJ (1982). New bacteriophage typing system forYersinia enterocolitica, Yersinia kristensenii, Yersinia frederiksenii, andYersinia intermedia: correlation with serotyping, biotyping, and antibiotic susceptibility. J Clin Microbiol 15:491–502
Bercovier H, Ursing J, Brenner DJ, Steigerwalt AG, Fanning GR, Carter GP, Mollaret HH (1980)Yersinia kristensenii: a new species of Enterobacteriaceae composed of sucrose-negative strains (formerly called atypicalYersinia enterocolitica orYersinia enterocolitica-like). Curr Microbiol 4:219–224
Brenner DJ, Ursing J, Bercovier H, Steigerwalt AG, Fanning GR, Alonso JM, Mollaret HH (1980) Deoxyribonucleic acid relatedness inYersinia enterocolitica andYersinia enterocolitica-like organisms. Curr Microbiol 4:195–200
Fukushima H (1985) Direct isolation ofYersinia enterocolitica andYersinia pseudotuberculosis from meat. Appl Environ Microbiol 50:710–712
Fukushima H (1987) New selective agar medium for isolation of virulentYersinia enterocolitica. J Clin Microbiol 25:1068–1073
Fukushima H, Tsubokura M (1985)Yersinia enterocolitica biotype 3B serotype 03 phage type II infection in pigs. Jpn J Vet Sci 47:1011–1015
Fukushima H, Tsubokura M, Otsuki K, Kawaoka Y (1984) Biochemical heterogeneity of serotype 03 strains of 700Yersinia strains isolated from humans, other animals, flies, animal feed, and river water. Curr Microbiol 11:149–154
Higuchi K, Smith JL (1961) Studies on the nutrition and physiology ofPasteurella pestis. VI. A differential plating medium for the estimation of the mutation rate to avirulence. J Bacteriol 81:605–608
Kandolo K, Wauters G (1985) Pyrazinamidase activity inYersinia enterocolitica and related organisms. J Clin Microbiol 21:980–982
Kaneko K, Maruyama T (1987) Pathogenicity ofYersinia enterocolitica serotype 03 biotype 3 strains. J Clin Microbiol 25:454–455
Laird WJ, Cavanaugh DC (1980) Correlation of autoagglutination and virulence of yersiniae. J Clin Microbiol 11:430–432
Maruyama T (1987)Yersinia enterocolitica infection in humans and isolation of the microorganisms from pigs in Japan. Contrib Microbiol Immunol 9:48–55
Shiozawa K, Akiyama M, Sahara K, Hayashi M, Nishina T, Murakami M, Asakawa Y (1987) Pathogenicity ofYersinia enterocolitica biotype 3B and 4, serotype 0∶3 isolates from pork samples and humans. Contrib Microbiol Immunol 9:30–40
Wauters G, Kandolo K, Janssens M (1987) Revised biogrouping scheme ofYersinia enterocolitica. Contrib Microbiol Immunol 9:14–21
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Fukushima, H., Maruyama, K., Omori, I. et al. Isolation of sucrose-negativeYersinia enterocolitica biotype 3 serotype 03 strains and their pathogenicity. Current Microbiology 17, 199–202 (1988). https://doi.org/10.1007/BF01589452
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DOI: https://doi.org/10.1007/BF01589452