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Iron uptake byDesulfovibrio vulgaris outer membrane components in artificial vesicles

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Abstract

Desulfovibrio vulgaris lipopolysaccharide and outer membrane proteins (OMPs) were incorporated into vesicles ofD. vulgaris phospholipid and studied for [55Fe]binding activity. Both lipopolysaccharide and an extract of two major OMPs caused large increases in55Fe uptake over control (phospholipid only) vesicles. CommercialSalmonella typhimurium lipopolysaccharide gave a similar result, but the effect was inhibited by calcium ions; this was not the case forDesulfovibrio. The lipid A portion ofS. typhimurium lipopolysaccharide had a high iron-binding ability, whereasDesulfovibrio lipid A iron binding was little different from control values;D. vulgaris lipopolysaccharide thus has a specific iron-binding site within its polysaccharide side chain.

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  1. G. Bradley

    Present address: Department of Biological Sciences, Plymouth Polytechnic, Drake Circus, PL4 8AA, Plymouth, Devon, UK

Authors and Affiliations

  1. Department of Biological Sciences, City of London Polytechnic, Old Castle Street, E1 7NT, London, England

    G. Bradley &  C. C. Gaylarde

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  1. G. Bradley
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  2. C. C. Gaylarde
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Bradley, G., Gaylarde, C.C. Iron uptake byDesulfovibrio vulgaris outer membrane components in artificial vesicles. Current Microbiology 17, 189–192 (1988). https://doi.org/10.1007/BF01589450

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  • DOI: https://doi.org/10.1007/BF01589450

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