Abstract
A subcellular fractionation study was carried out onStreptococcus mitis ATCC 9811 to determine the location of the arginine deiminase pathway. Arginine deiminase activity was detected in the cell wall fraction, ornithine carbamoyltransferase activity was recovered in both the cell wall and cytoplasmic fractions, and carbamate kinase activity was detected in the cytoplasmic fraction.
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Literature Cited
Abdelal AT (1979) Arginine catabolism by microorganisms. Annu Rev Microbiol 33:139–168
Crow VL, Thomas TD (1982) Arginine metabolism in lactic streptococci. J Bacteriol 150:1024–1032
Delisle AL, Weaver DS (1981) Effects of high concentrations ofl-arginine on acid production, cell yield and viability of the oral bacteriumStreptococcus mutans BHT. Arch Oral Biol 26:693–696
Ferro KJ, Bender GR, Marquis RE (1983) Coordinately repressible arginine deiminase system inStreptococcus sanguis. Curr Microbiol 9:145–150
Fiske CH, SubbaRow Y (1925) The colorimetric determination of phosphorus. J Biol Chem 66:375–400
Flammann HT, Weckesser J (1984) Characterization of the cell wall and outer membrane ofRhodopseudomonas capsulata. J Bacteriol 159:191–198
Fujita M, Matsui H, Nagano K, Nakao M (1971) Asymmetric distribution of ouabain-sensitive ATPase activity in rat intestinal mucosa. Biochim Biophys Acta 233:404–408
Hamada S, Slade HD (1980) Biology, immunology, and cariogenicity ofStreptococcus mutans. Microbiol Rev 44:331–384
Hartree EF (1972) Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem 48:422–427
Hiraoka BY (1985) Immunochemical properties and intracellular localization of two molecular forms of arginine aminopeptidase inStreptococcus mitis ATCC 9811. Biochim Biophys Acta 841:166–172
Hiraoka BY, Fukasawa K, Harada M (1983) Purification and characterization of two novel arginine aminopeptidases fromStreptococcus mitis ATCC 9811. J Biochem 94:1201–1208
Hiraoka BY, Mogi M, Fukasawa K, Harada M (1986) Coordinate repression of arginine aminopeptidase and three enzymes of the arginine deiminase pathway inStreptococcus mitis. Biochem Int 12:881–887
Mercenier A, Simon J-P, Wauven CV, Haas D, Stalon V (1980) Regulation of enzyme synthesis in the arginine deiminase pathway ofPseudomonas aeruginosa. J Bacteriol 144:159–163
Oginsky EL (1957) Isolation and determination of arginine and citrulline. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol 3. New York: Academic Press
Schnaitman CA (1970) Protein composition of the cell wall and cytoplasmic membrane ofEscherichia coli. J Bacteriol 104:890–901
Simon J-P, Wargnies B, Stalon V (1982) Control of enzyme synthesis in the arginine deiminase pathway ofStreptococcus faecalis. J Bacteriol 150:1085–1090
Singer DL, Kleinberg I (1978) Ammonia and urea content of human incisor tooth plaque. Arch Oral Biol 2:119–125
Stalon V, Ramos F, Pierard A, Wiame J-M (1972) Regulation of the catabolic ornithine carbomoyltransferase ofPseudomonas fluorescens. Eur J Biochem 29:25–35
Wagner RC (1976) The effect of tannic acid on electron images of capillary endothelial cell membranes. J Ultrastruct Res 57:132–139
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Hiraoka, B.Y., Harada, M., Fukasawa, K. et al. Intracellular localization of the arginine deiminase pathway inStreptococcus mitis . Current Microbiology 15, 81–84 (1987). https://doi.org/10.1007/BF01589366
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DOI: https://doi.org/10.1007/BF01589366