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Demonstration ofChlamydia trachomatis in papanicolaou-stained gynecological smears

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A fluorescent antibody staining method was developed to detectChlamydia trachomatis elementary bodies in Papanicolaou-stained cervical smears. Twenty-five known chlamydia-positive and 12 chlamydia-negative smears were correctly identified using this method. Smears from individuals with culture-positive chlamydia infections showed a high density of elementary bodies. Among 64 routine cervical smears, 27% (6/22) of smears that showed inflammatory changes were positive by fluorescent antibody staining for chlamydia, compared to 2.4% (1/42) of smears that were negative for inflammatory changes. This method should facilitate detection of chlamydia infections in populations undergoing cytological screening.

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Rantala, I., Kivinen, S. Demonstration ofChlamydia trachomatis in papanicolaou-stained gynecological smears. Eur. J. Clin. Microbiol. Infect. Dis. 17, 46–48 (1998).

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