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Cloning and sequence analysis of the flagellin gene ofCampylobacter jejuni TGH9011

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Abstract

Flagella are essential for motility and have been implicated to be one of the pathogenic determinants. The flagellum ofCampylobacter jejuni is a polymeric structure of a 62-kd protein. Using a high-affinity flagellin antibody to screen a lambda gt 11 phage genomic expression library ofC. jejuni strain TGH9011 (Serotype LIO36), a recombinant phage clone lambda gt 11RK that expresses theC. jejuni flagellin protein was isolated. The recombinant lambda gt 11 RK produced a 56-kd protein upon induction with isopropylthiogalactoside, which reacted specifically with anti-flagellin antibody. The flagellin gene was sequenced, and comparative analysis of the nucleotide and amino acid sequence identified a region of the flagellin that shows hypervariability among differentCampylobacter species and strains.

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Authors and Affiliations

  1. Department of Microbiology, University of Toronto, Fitzgerald Building, 150 College St., M5S-1A8, Toronto, Ontario, Canada

    Rubina Khawaja, Kuldeep Neote, Hermine L. Bingham, John L. Penner &  Voon L. Chan

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  1. Rubina Khawaja
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  2. Kuldeep Neote
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  3. Hermine L. Bingham
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  4. John L. Penner
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  5. Voon L. Chan
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Khawaja, R., Neote, K., Bingham, H.L. et al. Cloning and sequence analysis of the flagellin gene ofCampylobacter jejuni TGH9011. Current Microbiology 24, 213–221 (1992). https://doi.org/10.1007/BF01579284

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