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Mitochondrial plasmid-like molecules in fertile and male sterileVicia faba L

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Abstract

Biochemical analysis and electron microscopy showed that mitochondria of both the fertile and the male sterile 350 and 447 cytoplasms ofVicia faba. L. contain two small supercoiled DNA molecules of mean length of 1 700 and 1 420 base pairs in addition to the main mitochondrial DNA of high molecular weight. By agarose gel electrophoresis, the male sterile cytoplasm 350 is distinguished from the fertile cytoplasm and from the male sterile cytoplasm 447 by the presence of an additional supercoiled DNA molecule of approximately 1 540 bp.

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References

  1. Boutry M, Faber A-M, Gillis F, Briquet M: Mitochondrial modification in two cytoplasmic male-sterile line of Vicia faba. Arch Int Physiol Biochim 89:151, 1981.

    Google Scholar 

  2. Boutry, M, Briquet M: Mitochondrial modifications associated with the cytoplasmic male sterility in Faba beans. Eur J Biochem 127:129–135, 1982.

    Google Scholar 

  3. Collins RA, Stohl LL, Coole MD, Lambowitz AM: Characterization of a novel plasmid DNA found in mitochondria of N. crassa. Cell 24:443–452, 1981.

    Google Scholar 

  4. Dale RMK: Sequence homology among different, size classes of plant mt DNAs. Proc Natl Acad Sci USA 78:4453–4457, 1981.

    Google Scholar 

  5. Dale RMK: Structure of plant mitochondrial DNAs. In Slonimski P, Borst P, Attardi G (eds) Mitochondrial Genes. Cold Spring Harbor Laboratory, New York, pp. 471–476, 1982.

    Google Scholar 

  6. Davis RW, Simon M, Davidson N: Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods Enzymol 21:413–428, 1971.

    Google Scholar 

  7. Dixon LK, Leaver CJ: Mitochondrial, gene expression and cytoplasmic male sterility in sorghum. Plant Mol Biol 1:89–102, 1982.

    Google Scholar 

  8. Kemble RJ, Bedbrook JR: Low molecular weight circular and linear DNA in mitochondria from normal and male-sterile Zea mays cytoplasm. Nature 284:565–566, 1980.

    Google Scholar 

  9. Kück U, Stahl U, Esser K: Plasmid-like DNA is part of mitochondrial DNA in Podospora anserina. Curr Genet 3:151–156, 1981.

    Google Scholar 

  10. Levings III CS, Kim BD, Pring, DR, Conde MF, Mans RJ, Laughman, JR, Cabay-Laughnam SJ: Cytoplasmic reversion of cms-S in Maize:. association with a transpositional event. Science 209:1021–1023, 1980.

    Google Scholar 

  11. Negruk VI, Cherny, DI, Nikiforova, ID, Aleksandrov AA, Butenko RG: Isolation and characterization of minicircular DNAs found in mitochondrial fraction of Vicia faba. FEBS Lett 142:115–117, 1982.

    Google Scholar 

  12. Palmer JD, Shields CR, Cohen DB, Orton TJ: An unusual mitochondrial DNA plasmid in the genus Brassica. Nature 301:725–728, 1983.

    Google Scholar 

  13. Powling A: Species of small DNA molecules found in mitochondria from sugarbeet with normal and male sterile cytoplasms. Mol gen Genet 183:82–84, 1981.

    Google Scholar 

  14. Pring DR, Levings, III CS, Hu WWL, Timothy, DH: Unique DNA associated with mitochondria in the ‘S’-type cytoplasm of male sterile maize. Proc Natl Acad Sci USA 74:2904–2908, 1977.

    Google Scholar 

  15. Pring DR, Conde MF, Schertz, KF, Levings III CS: Plasmid-like DNAs associated with mitochondria of cytoplasmic male-sterile Sorghum. Mol gen Genet 186:180–184, 1982.

    Google Scholar 

  16. Sutcliff JG: pBr 322 restriction map derived from the DNA sequence: accurate DNA size markers up to 436 1 nucleotide pairs Long. Nucl Acids Res 5:2721–2728, 1978.

    Google Scholar 

  17. Tudzynski P, Esser K: Chromosomal and extrachromosomal control of senescence in the ascomycete Podospora anserina. Mol gen Genet 173:71–84, 1979.

    Google Scholar 

  18. Tudzynski P, Düvell A, Esser K: Extrachromosmal genetics of Claviceps purpurea. Curr Genet 7:145–150, 1983.

    Google Scholar 

  19. Weissinger AK, Timothy DH, Levings III CS, Hu WWL, Goodman MM: Unique plasmid-like mitochondrial DNAs from indigenous maize races of Latin America. Proc Natl Acad Sci USA 79:1–5, 1982.

    Google Scholar 

  20. Wiegand, RC, Godson GN, Radding CM: Specificity of the S1 nuclease from Aspergillus oryzae. J Biochem 250:8848–8855, 1975.

    Google Scholar 

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Goblet, JP., Boutry, M., Duc, G. et al. Mitochondrial plasmid-like molecules in fertile and male sterileVicia faba L. Plant Mol Biol 2, 305–309 (1983). https://doi.org/10.1007/BF01578591

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  • DOI: https://doi.org/10.1007/BF01578591

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