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Molecular cloning of pea mRNAs encoding a shoot-specific polypeptide and light-induced polypeptides

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Summary

The molecular cloning of cDNA corresponds to pea seedling mRNA sequences encoding a shoot-specific polypeptide, the small subunit of the ribulose 1,5 biphosphate carboxylase and a component of the light-harvesting chlorophyll a/b complex is described. cDNA prepared from polysomal poly(A)RNA of light-grown shoots was enriched for shoot-specific and light-induced sequences by heterologous liquid hybridization with mercurated polysomal poly(A)RNA of dark-grown roots, followed by sulfhydryl chromatography. Cloned shoot-specific sequences were identified by 2D electrophoretic analysis of hybrid release translation products. The cloned shoot-specific sequence corresponded to a mRNA of 850 nt present both in light-and dark-grown shoots, and produced anin vitro translation product of Mr27 500 and isoelectric point of 4.7.

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Authors and Affiliations

  1. Department of Developmental Plant Biology, Biological Centre, State University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands

    Sacco C. De Vries, Marco C. Harmsen, Martin T. R. Kuiper, Hans J. M. Dons & Joseph G. H. Wessels

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  1. Sacco C. De Vries
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  2. Marco C. Harmsen
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  3. Martin T. R. Kuiper
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  4. Hans J. M. Dons
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  5. Joseph G. H. Wessels
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De Vries, S.C., Harmsen, M.C., Kuiper, M.T.R. et al. Molecular cloning of pea mRNAs encoding a shoot-specific polypeptide and light-induced polypeptides. Plant Mol Biol 2, 295–303 (1983). https://doi.org/10.1007/BF01578590

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  • DOI: https://doi.org/10.1007/BF01578590

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