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Methods for culture ofBombyx mori wing discs

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Journal of tissue culture methods

Summary

A culture method forBombyx wing discs is described. Grace's insect medium,Bombyx hemolymph, and moderate doses of ecdysterone (0.02 to 0.1 µg/ml) were necessary for complete imaginal differentiation ofBombyx wing discs. This procedure is useful for understanding ecdysteroid action on insect imaginal differentiation and metamorphosis.

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Authors and Affiliations

  1. Faculty of Agriculture, Utsunomiya University, Mine 350, Utsunomiya, Tochigi, Japan

    Hideki Kawasaki

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  1. Hideki Kawasaki
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Kawasaki, H. Methods for culture ofBombyx mori wing discs. Journal of Tissue Culture Methods 12, 31–33 (1989). https://doi.org/10.1007/BF01578005

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  • DOI: https://doi.org/10.1007/BF01578005

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