Summary
Nutrient liposomes that incorporate peptones instead of serum proteins can be used for the lipid supplementation of insect cells in lieu of or in addition to vertebrate serum to enhance the growth or differentiation of specific insect cell types in culture. The technique described here for the preparation of nutrient peptoliposomes involves the combination of both natural and synthetic phosphatidyl cholines (containing essential polyunsaturated fatty acids) with appropriate sterols. These are protected against premature oxidation by antioxidants (glutathione, alanine, protein hydrolysates) and antioxidant synergists (citrate, ascorbate, plus other amino acids) in the basal tissue culture medium as well as in the liposomal preparation itself (alphatocopherol acetate and protein hydrolysates). The lipid components are filter sterilized in chloroform and then dried before aqueous sonication, allowing the subsequent formation of a random array of unsized uni- and multilamellar liposomes, which are suitable as direct suppliers of lipid nutrients as well as nutrient carriers for lipid-soluble supplements.
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Goodwin, R.H. Construction of peptoliposomes for the incorporation of nutrient lipid supplements in insect cell culture media. Journal of Tissue Culture Methods 12, 17–20 (1989). https://doi.org/10.1007/BF01578002
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DOI: https://doi.org/10.1007/BF01578002