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Transfection of Lepidopteran insect cells with Baculovirus DNA

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Journal of tissue culture methods

Summary

Several protocols are presented for preparation and transfection of Baculovirus and plasmid DNAs into Lepidopteran insect cells using the calcium-phosphate co-precipitation technique. Important parameters for optimum efficiency include the inherent susceptibility of the recipient cell line for transfection, and the method of preparation of viral and plasmid DNAs. The protocols presented provide reproducible high efficiencies for transfection of several Lepidopteran cell lines.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Notre Dame, 46556, Notre Dame, Indiana

    Bartholomew G. Corsaro & M. J. Fraser

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  1. Bartholomew G. Corsaro
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  2. M. J. Fraser
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Corsaro, B.G., Fraser, M.J. Transfection of Lepidopteran insect cells with Baculovirus DNA. Journal of Tissue Culture Methods 12, 7–11 (1989). https://doi.org/10.1007/BF01578000

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  • DOI: https://doi.org/10.1007/BF01578000

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