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Fimbriation ofEscherichia coli in urinary tract infections. Comparisons between bacteria in the urine and subcultured bacterial isolates

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Abstract

Midstream urine samples from 37 patients with urinary tract infections were studied by electron microscopy, hemagglutination, and the salt aggregation test (SAT) to measure the hydrophobicity of the bacterial surface.Escherichia coli subcultured from these urine samples were tested in the same way. Fimbriae were visualized onE. coli in the urine of 31 specimens, and all these urines containedE. coli that expressed pronounced surface hydrophobicity and aggregated in ammonium sulfate of 0.1–1.6 M final concentration. Hemagglutination of human and/or guinea pig erythrocytes was expressed by 21E. coli in the urine. TheE. coli strains subcultured from these 31 urine samples were also fimbriated, but the number of fimbriae per bacterium as well as the percentage of fimbriated bacteria varied compared with the directly collected strains. The surface hydrophobicity and hemagglutination were similar to the results with the directly collected bacteria. However, after serial transfer in CFA-broth under static conditions, all non-hemagglutinating strains expressed mannose-sensitive hemagglutination of guinea pig erythrocytes, and three strains also expressed weak mannose-resistant hemagglutination of human erythrocytes. Following serial transfer, fimbriae were also visualized on the sixE. coli strains that appeared non-fimbriate in the urine. It is thus concluded thatE. coli causing urinary tract infection are often fimbriated and express surface hydrophobicity in the urine. Based on these findings, a rapid method to isolate hydrophobic, possibly fimbriated bacteria was tried in which the urine was mixed with a hydrophobic gel. Hydrophobic bacteria bound to the gel and could be eluted from the sedimented gel.

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Literature Cited

  1. Bar-Shavit, Z., Goldman, R., Ofek, I., Sharon, N., Mirelman, D. 1980. Mannose-binding activity ofEscherichia coli: a determinant of attachment and ingestion of the bacteria by macrophages. Infection and Immunity29:417–424.

    Google Scholar 

  2. Björkstén, B., Wadström, T. 1982. Interaction of fimbriatedEscherichia coli with polymorphonuclear leucocytes. Infection and Immunity,38:298–305.

    Google Scholar 

  3. Brinton, C. C., Jr. 1977. The piliation phase syndrome and the uses of purified pili in disease control, pp. 33–70. In: Proceedings of the 13th Joint Conference on Cholera. Department of Health, Education and Welfare Publication No 78-1590. Bethesda: National Institutes of Health.

    Google Scholar 

  4. Chick, S., Harber, M. J., Mackenzie, R., Asscher, A. W. 1981. Modified method for studying bacterial adhesion to isolated uroepithelial cells and uromucoid. Infection and Immunity34:256–261.

    Google Scholar 

  5. Duguid, J. P., Clegg, S., Wilson, M. I. 1979. The fimbrial and non-fimbrial haemagglutinins ofEscherichia coli. Journal of Medical Microbiology12:213–227.

    Google Scholar 

  6. Duguid, J. P., Old, D. C. 1980. Adhesive properties of Enterobacteriaceae, pp. 186–217. In: Beachey, E. H. (ed.) Bacterial adherence. Receptors and Recognition Series B, Volume 6. London: Chapman and Hall.

    Google Scholar 

  7. Evans, D. G., Evans, D. J., Jr, Tjoa, W. 1977. Hemagglutination of human group A erythrocytes by enterotoxigenicEscherichia coli isolated from adults with diarrhea: correlation with colonization factor. Infection and Immunity18:330–337.

    Google Scholar 

  8. Faris, A., Wadström, T., Freer, J. 1981. Hydrophobic, adsorptive and hemagglutinating properties ofEscherichia coli possessing colonization factor antigens (CFA/I or CFA/II), type 1 pili and other pili. Current Microbiology5:67–72.

    Google Scholar 

  9. Green, C. P., Thomas, V. L. 1981. Hemagglutination of human type O erythrocytes, hemolysin production and serogrouping ofEscherichia coli isolates from patients with acute pyelonephritis, cystitis and asymptomatic bacteriuria. Infection and Immunity31:309–315.

    Google Scholar 

  10. Hagberg, L., Jodal, U., Korhonen, T., Lindin-Jansson, G., Lindberg, U., Svanborg Edén, C. 1981. Adhesion, hemagglutination and virulence ofEscherichia coli causing urinary tract infections. Infection and Immunity31:564–570.

    Google Scholar 

  11. Harber, M. J., Chick, S., Mackenzie, R., Asscher, A. W. 1982. Lack of adherence ot epithelial cells by freshly isolated urinary pathogens. LancetI:586–588.

    Google Scholar 

  12. Kuch, B., Pál, T., Emödy, L. 1982. Bacterial adherence and urinary tract infection. LancetII:107.

    Google Scholar 

  13. Källenius, G., Möllby, R., Svensson, S. B., Winberg, J., Lundbald, A., Svensson, S., Cedergren, B. 1980. The Pk antigen as receptor for the hemagglutinin of pyelonephriticEscherichia coli. FEMS Microbiology Letters7:297–302.

    Google Scholar 

  14. Leffler, H., Svanborg-Edén, C. 1980. Chemical identification of a glycosphingolipid receptor forEscherichia coli attaching to urinary tract epithelial cells and agglutinating human erythrocytes. FEMS Microbiology Letters8:127–134.

    Google Scholar 

  15. Lindahl, M., Faris, A., Wadström, T., Hjertén, S. 1981. A new test based on “salting out” to measure relative surface hydrophobicity of bacterial cell. Biochimica Biophysica Acta677:471–476.

    Google Scholar 

  16. Ljungh, Å., Wadström, T. 1982. Salt aggregation test for measuring cell surface hydrophobicity of urinaryEscherichia coli. European Journal of Clinical Microbiology1:388–393.

    Google Scholar 

  17. Marrie, T. J., Lam, J., Costerton, J. W. 1980. Bacterial adhesion to uroepithelial cells: A morphological study. Journal of Infectious Diseases142:239–246.

    Google Scholar 

  18. Mäkelä, P. H., Korhonen, T. K. 1982. Bacterial adherence and urinary tract infection. LancetI:961.

    Google Scholar 

  19. Ofek, I., Mosek, A., Sharon, N. 1981. Mannose-specific adherence ofEscherichia coli freshly excreted in the urine of patients with urinary tract infections and of isolates subcultured from the infected urine. Infection and Immunity34:708–711.

    Google Scholar 

  20. Ørskov, I., Ferenc, A., Ørskov, F. 1980. Tamm-Horsfall protein or uromucoid is the normal urinary slime that traps type I fimbriatedEscherichia coli. LancetI:887.

    Google Scholar 

  21. Schwartz, J., Menoret, P., Begue, P., Lasfargues, G. 1982. Bacterial adherence and urinary tract infection. LancetII:108.

    Google Scholar 

  22. Sjoblad, R. D., Doetsch, R. N. 1982. Adsorption of polarly flagellated bacteria to surfaces. Current Microbiology7:191–194.

    Google Scholar 

  23. Svanborg-Edén, C., Hagberg, L., Hansson, L. Å., Lomberg, H., Ørskov, I., Ørskov, F. 1982. Bacterial adherence and urinary tract infection. LancetII:962.

    Google Scholar 

  24. Wadström, T., Trust, T. J. 1983. Bacterial lectins. Medical Microbiology, in press.

  25. Väisänen, V., Elo, J., Tallgren, L. G., Siitonen, A., Mäkelä, P. H., Svanborg-Edén, C., Källenius, G., Svensson, S. B., Hultberg, H., Korhonen, T. 1981. Mannose-resistant haemagglutination and P antigen recognition are characteristic ofEscherichia coli causing primary pyelonephritis. LancetII:1366–1369.

    Google Scholar 

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Authors and Affiliations

  1. Stockholm County Council Central Microbiological Laboratory, Stockholm

    Åsa Ljungh

  2. Department of Bacteriology and Epizootology, Biomedicum, Uppsala, Sweden

    Torkel Wadström

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  1. Åsa Ljungh
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  2. Torkel Wadström
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Ljungh, Å., Wadström, T. Fimbriation ofEscherichia coli in urinary tract infections. Comparisons between bacteria in the urine and subcultured bacterial isolates. Current Microbiology 8, 263–268 (1983). https://doi.org/10.1007/BF01577725

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