Summary
Extracted grape waster material and pressed apple pulp were tested as carbon sources forPenicillium funiculosum 515,Myrothecium verrucaria 9095 andAspergillus niger TMF-15. They were good growth substrates, especially forA. niger. When cultivated on mixed substrate in optimized nutrient medium,A. niger accumulated a product of 35% crude protein with a maximum productivity of 0.117 g protein/1/h and cellulose consumption of 90.92%.A. niger also produced the highest levels of cellulase activity. Maximum carboxymethyl cellulase and activity against filter paper were 494 units/l and 97 units/l, respectively.
Similar content being viewed by others
References
Bajracharya, R. and R.E. Mudgett. 1979. Solid-substrate fermentation of alfalfa for enhanced protein recovery. Biotechnol. Bioeng. 21: 551–560.
Bull, A.T. and H. Slater. 1982. Microbial Interaction and Communities. Academic Press, London.
Bushell, M.E. and J.H. Slater. 1981. Mixed Culture Fermentation. Academic Press, London.
Edwards, V.H. and C.B. Wilke. 1968. Mathematical representation of batch culture data. Biotechnol. Bioeng. 10: 205–232.
Ghose, T.H. 1984. Measurement of Cellulase Activities. Commission on Biotechnology, IUPAC, New Delhi.
Grant, J. 1965. Laboratory Handbook of Cellulase and Paper Production (In Russian), pp. 17–22, Lesnaya promishlenost, Moscow.
Kalininskaya, T.A. and T.T. Kultyshina. 1974. Application of the anthrone method to measure cellulose consumption in soil. Prikl. Biohim. Microbiol. 10: 618–620.
Kuzmanova, S., A. Dimitrovski and E. Vandeska. 1987. Kinetic parameters of pectinase production. 2nd Yugoslav Congress on Chemical Engineering, 11–15 May, Dubrovnik.
Mandels, M., and J. Weber. 1969. The production of cellulases. Adv. Chem. Ser. 95: 391–414.
Miller, G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426–428.
Moo-Young, M., D.S. Chahal and D. Vlach. 1978. Single cell protein from various chemically pretreated wood substrates usingChaetomium cellulolyticum. Biotechnol. Bioeng. 29: 107–118.
Peitersen, N. 1975. Production of cellulases and protein from barley straw byTrichoderma viride. Biotechnol. Bioeng. 17: 361–374.
Rolz, C. 1984. Microbial biomass from renewables: a second review of alternatives. In: Annual Reports on Fermentation Processes, Vol. 7, pp. 215–358. Academic Press, Orlan.
Updegraff, D.M. 1971. Utilization of cellulose from waste paper byMyrothecium verrucaria. Biotechnol. Bioeng. 13: 77–97.
Vandeska, E., S. Kuzmanova, A. Dimitrovski and D. Doneva. 1989. Optimization of culture medium for single cell protein production. Prehrambeno-technol. biotechnol. rev. 27: 48.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kuzmanova, S., Vandeska, E. & Dimitrovski, A. Production of mycelial protein and cellulolytic enzymes from food wastes. Journal of Industrial Microbiology 7, 257–261 (1991). https://doi.org/10.1007/BF01577653
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01577653