Abstract
Cyanophycin granule polypeptide (CGP) formation and degradation was studied in a facultatively heterotrophic, nitrogen-fixing cyanobacterium. CGP was synthesized primarily under photoautotrophic growth conditions in nitrogen-fixing cells. Addition of a utilizable carbon source stopped CGP synthesis, the content of which declined in cultures as the cells grew heterotrophically in light or dark. A high-performance liquid chromatographic method was developed to determine CGP in cyanobacterial extracts and permitted analyses of CGP and simultaneous examination of the protein profile of the cells. The data indicated that, once formed in the photoautotrophs, CGP was not degraded, even by cells in which extensive protein breakdown was occurring.
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Literature Cited
Allen MM, Hawley MA (1983) Protein degradation and synthesis of cyanophycin granule polypeptide inAphanocapsa sp. J Bacteriol 154:1480–1484
Allen MM, Hutchison F, Weathers PJ (1980) Cyanophycin granule polypeptide formation and degradation in the cyanobacteriumAphanocapsa 6308. J Bacteriol 141:687–693
Allen MM, Morris R, Zimmerman W (1984) Cyanophycin granule polypeptide protease in a unicellular cyanobacterium. Arch Microbiol 138:119–123
Bietz JA (1983) Separation of cereal proteins by reversed phase high performance liquid chromatography. J Chromatog 255:219–238
Boussiba S, Liu ZQ, Gibson J (1984) Endogenous ammonia production byAnacystis nidulans R2 induced by methionine sulfoximine. Arch Microbiol 138:217–219
Gupta M, Carr NG (1981) Enzyme activities related to cyanophycin metabolism in heterocysts and vegetative cells ofAnabaena spp. J Gen Microbiol 125:17–23
Hill DJ (1975) The pattern of development ofAnabaena in theAzolla-Anabaena symbiosis. Planta 122:179–184
Hill DJ (1977) The role ofAnabaena in theAzolla-Anabaena symbiosis. New Phytol 78:611–616
Lambert GR, Smith GD (1981) Hydrogen uptake by the nitrogen-starved cyanobacteriumAnabaena cylindrica. Arch Biochem Biophys 211:360–367
Lang NJ (1968) The fine structure of blue-green algae. Annu Rev Microbiol 22:15–46
Lang NJ, Simon RD, Wolk CP (1972) Correspondence of cyanophycin granules with structured granules inAnabaena cylindrica. Arch Microbiol 83:313–320
Lumpkin TA, Plucknett DL (1980)Azolla: botany, physiology and use as a green manure. Econ Bot 34:111–153
Newton JW (1985) Photosystem II and ammonia liberation by cyanobacteria. In: Evans HJ, Bottomley PT, Newton WE (eds) Nitrogen fixation research progress Dordrecht Martinus Nijhoff, p 434
Newton JW, Cavins JF (1985) Liberation of ammonia during nitrogen fixation by a facultatively heterotrophic cyanobacterium. Biochim Biophys Acta 809:44–50
Newton JW, Herman AI (1979) Isolation of cyanobacteria from the aquatic fern,Azolla. Arch Microbiol 120:161–165
Newton JW, Tyler DD (1984) Formation and breakdown ofAzolla polypeptides in paddy soils. In: Silver WS, Schroder EC (eds) Developments in plant and soil sciences, vol 13: Practical application ofAzolla for rice production. Dordrecht: Martinus Nijhoff and Junk, pp 17–28
Ownby JD, Shannahan M, Hood EB (1979) Protein synthesis and degradation inAnabaena during nitrogen starvation. J Gen Microbiol 110:255–261
Rippka R, Stanier RY (1978) The effects of anaerobiosis on nitrogenase synthesis and heterocyst development by Nostocacean cyanobacteria. J Gen Microbiol 105:83–94
Rippka R, Waterbury JB (1977) Anaerobic nitrogenase synthesis in nonheterocystous cyanobacteria. FEMS Microbiol Lett 2:83–86
Roger PA, Kulasooriya SA (1980) Blue green algae and rice. International Rice Research Inst, PO Box 933, Manila, Phillipines. ISBN 971-104-028X, 112 pp.
Simon RD (1971) Cyanophycin granules from the blue-green algaAnabaena cylindrica: a reserve material consisting of copolymers of aspartic acid and arginine. Proc Nat Acad Sci USA 68:265–267
Simon RD (1973) The effect of chloramphenicol on the production of cyanophycin granule polypeptide in the blue green algaAnabaena cylindrica. Arch Microbiol 92:115–122
Simon RD (1973) Measurement of the cyanophycin granule polypeptide contained in the blue green algaAnabaena cylindrica. J Bacteriol 114:1213–1216
Simon RD, Weathers P (1976) Determination of the structure of the novel polypeptide containing aspartic acid and arginine which is found in cyanobacteria. Biochim Biophys Acta 420:165–176
Stanier RY, Cohen-Bazire G (1977) Phototrophic procaryotes: the cyanobacteria. Annu Rev Microbiol 31:225–274
Stanier RY, Kunisawa R, Mandel M, Cohen-Bazire G (1971) Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev 35:171–205
Spector T (1978) Refinement of the Coomassie blue method of protein determination. Anal Biochem 86:142–146
Stevens SE, Paone DAM, Balkwill DL (1981) Accumulation of cyanophycin granules as a result of phosphate limitation inAgmenellum quadruplicatum. Plant Physiol 67:716–719
Stewart WDP, Rowell P (1975) Effects ofl-methionine-dl-sulfoximine on the assimilation of newly fixed NH3, acetylene reduction, and heterocyst production inAnabaena cylindrica. Biochem Biophys Res Commun 65:846–856
Sutherland JM, Herdman M, Stewart WDP (1979) Akinetetes of the cyanobacteriumNostoc PCC 7524 macromolecular composition, structure and control of differentiation. J Gen Microbiol 115:273–287
Watanabe A, Kiyohara T (1960) Decomposition of blue-green algae as effected by the action of soil bacteria. J Gen Appl Microbiol 5:175–179
Wood NB, Haselkorn R (1980) Control of phycobiliprotein proteolysis and heterocyst differentiation inAnabaena. J Bacteriol 141:1375–1385
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Newton, J.W., Tyler, D.D. Cyanophycin granule polypeptide in a facultatively heterotrophic cyanobacterium. Current Microbiology 15, 207–211 (1987). https://doi.org/10.1007/BF01577532
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DOI: https://doi.org/10.1007/BF01577532