Abstract
CloDF13 copy mutants that have their resolution site (crl) deleted accumulate as multimeric plasmid molecules in their host cells and are lost from severalEscherichia coli stains within 60 generations. Here we demonstrate that CloDF13cop3crl − mutants are stably maintained in theE.coli strain G668, although the plasmid copy number is not affected. Furthermore, we show that the stable maintenance of those plasmids is achieved even in the presence of multimeric molecules. Therefore, we conclude that a complete monomerization of multimeric molecules appears not to be a prerequisite for accurate partition of the plasmid molecules over daughter cells. The G668 strain may be applied as host for the stabilization of resolution-negative, unstable CloDF13 or related replicons.
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van Putten, A.J., de Lang, R., Süter, CP.P.M. et al. Escherichia coli host G668 stabilizes plasmids that are unstable in otherEscherichia coli strains. Current Microbiology 15, 199–202 (1987). https://doi.org/10.1007/BF01577530
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DOI: https://doi.org/10.1007/BF01577530