Abstract
An anaerobic syntrophic bacterial culture degrading benzoate was isolated from a river sediment. The syntrophic organism was grown in coculture in the presence of a hydrogenotrophic strain,Desulfovibrio fructosovorans orMethanospirillum hungatei. The G+C content of the syntrophic benzoate degrader determined by density gradient ultracentrifugation was similar to that ofSyntrophus buswellii (54.3%). A method ensuring the G+C% determination of syntrophic bacteria is presented.
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Fardeau, ML., Ollivier, B., Soubrane, A. et al. Determination of the G + C content of twoSyntrophus buswellii strains by ultracentrifugation techniques. Current Microbiology 26, 185–189 (1993). https://doi.org/10.1007/BF01577375
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DOI: https://doi.org/10.1007/BF01577375