Abstract
The heat shock response of three cyanobacterial strains,Anabaena sp. Strain PCC (paris Culture Collection) 7120,Plectonema boryanum Strain PCC 6306, andSynechococcus sp. Strain PCC 7942, was characterized by polyacrylamide gel electrophoresis.Anabaena produced 33 heat shock proteins,P. boryanum 35 proteins, andSynechoccus 19 proteins. The rapid response to heat shock was consistent for all three strains, although the number of time-dependent proteins varied. All strains developed thermotolerance when first pretreated with a sublethal heat shock and then challenged with a previously lethal temperature. A 30-min 30°C incubation was required between the heat shock and challenge forSynechococcus, but not forAnabaena andP. boryanum. Synechococcus cells required a higher challenge temperature (51° vs. 49°C) than the other two strains to destroy control cells that were not pretreated with a heat shock.
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Literature Cited
Allen MM (1968) Simple conditions for growth of unicellular blue-green algae on plates. J Phycol 4:1–4
Bhagwat A, Apte SK (1989) Comparative analysis of proteins induced by heat shock, salinity, and osmotic stress in the nitrogen-fixing cyanobacteriumAnabaena sp. Strain L-31. J Bacteriol 171:5187–5189
Borbely G, Suranyi G, Korcz A, Palfi Z (1985) Effect of heat shock on protein synthesis in the cyanobacteriumSynechococcus sp. Strain PCC 6301. J Bacteriol 161:1125–1130
Bunning VK, Crawford RG, Tierney JT, Peeler JT (1990) Thermotolerance ofListeria monocytogenes andSalmonella typhimurium after sublethal heat shock. Appl Environ Microbiol 56:3216–3219
Carper SW, Duffy JJ, Gerner EW (1987) Heat shock proteins in thermotolerance and other cellular processes. Cancer Res 47:5249–5255
Chitnis PR, Nelson N (1991). Molecular cloning of the genes encoding two chaperone proteins of the cyanobacteriumSynechocystis sp. PCC 6803. J Biol Chem 266:58–65
Craig EA (1985) The heat shock response. CRC Crit Rev Biochem 18:239–280
Hall BG (1983) Yeast thermotolerance does not require protein synthesis. J Bacteriol 156:1363–1365
Hurkman WJ, Tanaka CK (1986) Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis. Plant Physiol 81:802–806
Laszlo A (1988) The relationship of heat-shock proteins, thermotolerance, and protein synthesis. Exp Cell Res 178:401–414
Laszlo A, Li GC (1985) Heat resistant variants of Chinese hamster fibroblasts altered in expression of heat shock protein. Proc Natl Acad Sci USA 82:8029–8033
Li GC, Werb Z (1982) Correlation between synthesis of heat shock proteins and development of thermotolerance in Chinese hamster fibroblasts. Proc Natl Acad Sci USA 79:3218–3222
Lindquist S (1986) The heat shock response. Annu Rev Biochem 55:1151–1191
Lindquist S, Craig EA (1988) The heat-shock proteins. Annu Rev Genet 22:631–677
Mackey BM, Derrick C (1990) Heat shock protein synthesis and thermotolerance inSalmonella typhimurium. J Appl Bacteriol 69:373–383
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press
Morimoto RI, Tissieres A, Georgopoulos C (eds.) (1990) Stress proteins in biology and medicine. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press
Nicholson TR, Osborn W, Howe CJ (1987) Induction of protein synthesis in response to ultraviolet light, nalidixic acid and heat shock in the cyanobacteriumPhormidium laminosum. Fed Eur Biochem Soc 221:110–114
O'Farrell PZ, Goodman HM, O'Farrell PH (1977) High resolution two dimensional electrophoresis of basic as well as acidic proteins. Cell 12:1133–1142
Pelham HBR (1989) Heat shock and the sorting of luminal ER proteins. EMBO J 8:3161–3176
Plesset J, Palm C, McLaughlin CS (1982) Induction of heat shock proteins and thermotolerance by ethanol inSaccharomyces cerevisiae. Biochem Biophys Res Commun 108:1340–1345
Rothman JE (1989) Polypeptide chain binding proteins: catalysts of protein folding and related processes in cells. Cell 59:591–601
Suranyi G, Korez A, Palfi Z, Borberly G (1987) Effects of light deprivation on RNA synthesis, accumulation of guanosine 3′(2′)-diphosphate 5′-diphosphate, and protein synthesis in heat shockedSynechococcus sp. Strain PCC 6301, a cyanobacterium. J Bacteriol 169:632–639
Trent J, Osipiuk J, Pinkau T (1990) Acquired thermotolerance and heat shock in the extremely thermophilic archaebacterium,Sulfolobus sp. strain B12. J Bacteriol 172:1478–1484
VanBoegelen RA, Acton MA, Neidhardt FC (1987) Induction of the heat shock regulon does not produce thermotolerance inEscherichia coli. Genes Dev 1:525–531
Webb R, Reddy KJ, Sherman LA (1990) Regulation and sequence of theSynechococcus sp. strain PCC 7942groESL operon, encoding a cyanobacterial chaperonin. J Bacteriol 172:5079–5088
Yamamori T, Yura T (1982) Genetic control of heat shock protein synthesis and its bearing on growth and thermal resistance inEscherichia coli K-12. Proc Natl Acad Sci USA 79:860–864
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Blondin, P.A., Kirby, R.J. & Barnum, S.R. The heat shock response and acquired thermotolerance in three strains of cyanobacteria. Current Microbiology 26, 79–84 (1993). https://doi.org/10.1007/BF01577340
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DOI: https://doi.org/10.1007/BF01577340