Abstract
We have developed a practical and economical device that efficiently concentrates ciliated cells, such asTetrahymena pyriformis, by using only low-speed, non-damaging centrifugation. This is carried out with a simple device consisting of a microcentrifuge tube connected to the barrel of a 60-ml plastic syringe. This device allows the concentration of essentially all the cells into a small volume without damaging cell structures or reducing their viability.
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Calzone FJ, Angerer RC, Gorovski MA (1982) Regulation of protein synthesis inTetrahymena: isolation and characterization of polysomes by gel filtration and precipitation at pH 5.3. Nucleic Acid Res 10:2145–2161
David ET, Smith KE (1981) Preparation and analysis of polyribosomes isolated fromTetrahymena pyriformis. Comp Biochem Physiol 69B:213–222
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Lang, D., Gauthier, D. A simple device for the rapid concentration or isolation ofTetrahymena . Current Microbiology 26, 63–64 (1993). https://doi.org/10.1007/BF01577337
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DOI: https://doi.org/10.1007/BF01577337