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Comparison of treatments for the in vitro detachment of cellulolytic bacteria from ruminal digesta samples

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Abstract

Four treatments were applied to ruminal digesta samples in a randomized complete block design experiment to evaluate their effect on the extent of detachment of ruminal cellulolytic bacteria. The treatments were: blending with anaerobic dilution solution (ADS), blending with ADS after a 6-h cold incubation, blending with ADS+Tween 80, and blending with ADS +Tween 80 after a 6-h cold incubation. Compared with blending in ADs alone, significant increases (p<0.2) of 10% and 12% in the number of cellulolytic bacteria were observed with the 6-h cold and the 6-h cold plus Tween 80 treatments, respectively. Tween 80 had no effect on the number of cellulolytic bacteria released from ruminal digesta samples irrespective of chilling. It was concluded that these additional treatments afforded little value over blending in buffer alone in detaching cellulolytic bacteria from ruminal digesta.

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Authors and Affiliations

  1. Microbiology and Nutrition Research, The Upjohn Company, 7922-190-MR, Kalamazoo, Michigan, USA

    Jane A. Z. Leedle, Karen Barsuhn & Tala J. Butine

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  1. Jane A. Z. Leedle
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  2. Karen Barsuhn
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  3. Tala J. Butine
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Leedle, J.A.Z., Barsuhn, K. & Butine, T.J. Comparison of treatments for the in vitro detachment of cellulolytic bacteria from ruminal digesta samples. Current Microbiology 15, 129–131 (1987). https://doi.org/10.1007/BF01577259

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