Current Microbiology

, Volume 25, Issue 6, pp 347–351 | Cite as

Isolation and characterization of siderophore, with antimicrobial activity, fromAzospirillum lipoferum M

  • Samir Shah
  • Vaidehi Karkhanis
  • Anjana Desai

Abstract

Azospirillum lipoferum M was found to produce catechol-type of siderophores under iron-starved conditions. Chemical characterization of siderophores revealed the presence of salicylic acid, 2,3-dihydroxybenzoic acid (DHBA), and 3,5-DHBA conjugated with threonine and lysine. Siderophore production was found to be maximum after 28 h of growth. In addition to their established role in iron transport, the siderophores exhibited antimicrobial activity against various bacterial and fungal isolates.

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Literature Cited

  1. 1.
    Carbin JL, Bulen WA (1969) The isolation and identification of 2,3-dihydroxybenzoic acid and 2N,6N-di(2,3-dihydroxybenzol)l-lysine formed by iron deficientAzotobacter vinelandii. Biochemistry 8:757–762Google Scholar
  2. 2.
    Dobereiner J, Day JM (1976) Associative symbiosis in tropical grasses: characterization of microorganisms and nitrogen fixing sites. In: Newton WE, Nyman CJ (eds) Proceedings of the First International Symposium on Nitrogen Fixation. Pullman: Washington State University Press, pp 518–536Google Scholar
  3. 3.
    Jadhav RS, Desai AJ (1992) Isolation and characterization of siderophore from cowpeaRhizobium (peanut isolate). Curr Microbiol 24:137–141Google Scholar
  4. 4.
    Joseph MV, Desai JD, Desai AJ (1983) Production of antimicrobial and bacteriocin-like substances byRhizobium trifolii. Appl Env Microbiol 45:532–535Google Scholar
  5. 5.
    Leong SA, Neilands JB (1982) Siderophore production by phytopathogenic microbial species. Arch Biochem Biophys 218:351–359Google Scholar
  6. 6.
    Modi MV, Shah KS, Modi VV (1986) Isolation and characterization of catechol like siderophore from cowpeaRhizobium. Arch Microbiol 141:156–158Google Scholar
  7. 7.
    Murray MY (ed) Comprehensive biochemistry, vol. 3. Oxford, UK: Pergamon, pp. 275–279.Google Scholar
  8. 8.
    Neilands JB (1981) Microbial iron compounds. Annu Rev Biochem 50:715–731Google Scholar
  9. 9.
    Neilands JB (1982) Microbial envelope proteins related to iron. Annu Rev Microbial 36:285–309Google Scholar
  10. 10.
    Patel U, Baxi MD, Modi VV (1988) Evidence for the involvement of iron siderophore in the transport of molybdenum in cowpeaRhizobium. Curr Microbiol 17:179–182Google Scholar
  11. 11.
    Payne SM, Lowler K (1983) Expression of hydroxamate and phenolate siderophores byShigella flexneri. J Bacteriol 155:949–955Google Scholar
  12. 12.
    Reeves M, Pine L, Neilands JB, Bullows A (1983) Absence of siderophore activity inLegionella sp. grown in iron deficient media. J Bacteriol 154:324–329Google Scholar
  13. 13.
    Rogers HJ, Woods VE, Synge C (1982) Antibacterial effect of Scandium and Indium complexes of enterochelin ofE. coli. J Gen Microbiol 128:2389–2394Google Scholar
  14. 14.
    Roslycky EB (1965) Losogeny in the rhizobia bacteria. Can Soc Microbiol, 15th annual meeting, pp 47–48Google Scholar
  15. 15.
    Saxena B, Modi M, and Modi VV (1986) Isolation and characterization of siderophore fromAzospirillum lipoferum D2. J Gen Microbiol 132:2219–2224Google Scholar
  16. 16.
    Saxena B, vithalani L, Modi VV (1989) Siderophore-mediated transport of molybdenum inAzospirillum lipoferum D2. Curr Microbiol 19:291–295Google Scholar
  17. 17.
    Tapia-Hernandez A, Mascarua-Esparza MA, Caballero-Mellado J. (1990) Production of bacteriocins and siderophorelike activity byAzospirillum brasilense. Microbios 64:73–83Google Scholar
  18. 18.
    Tarrand JJ, Krieg NR, Dobereiner J (1978) A taxonomic study of theSpirillum lipoferum group with descriptions of a new genus,Azospirillum gen nov and two speciesA. lipoferum (Beijerinki), comb. nov. andA. brasilense sp. nov. Can J Microbiol 24:967–980Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1992

Authors and Affiliations

  • Samir Shah
    • 1
  • Vaidehi Karkhanis
    • 1
  • Anjana Desai
    • 1
  1. 1.Department of Microbiology & Biotechnology Centre, Faculty of ScienceM.S. University of BarodaBarodaIndia

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