Abstract
The possibility thatChlamydia trachomatis contains peptidoglycan was examined by three methods. Preincubation of chlamydia with enzymes known to cleave peptidoglycan had no adverse effect on the subsequent development. Immunofluorescence studies with antistreptococcal peptidoglycan antisera failed to show any cross reactions with chlamydial antigens. The antichlamydial activity of anti-cell-wall antimicrobials was examined; β lactams proved the most active, and cycloserine and bacitracin also showed antichlamydial activity. Alaphosphin, phosphomycin, and vancomycin showed no antichlamydial activity at the concentrations tested.
Similar content being viewed by others
Literature Cited
Barbour, A. G., Amano, K., Hackstadt, T., Perry, L., Caldwell, H. O. 1982.Chlamydia trachomatis has penicillin binding proteins but not detectable muranic acid. Journal of Bacteriology151:420–428.
Brown, K. N., Percival, A. 1978. Penetration of antimicrobials into tissue culture cells and leucocytes. Scandinavian Journal of Infectious Diseases, Supplement14:251–260.
Edwards, D. 1980. Antimicrobial drug action. London: Mac-Millan.
Garret, A. J., Harrison, M. J., Manire, G. P. 1974. A search for the bacterial mucopeptide, muramic acid, inChlamydia. Journal of General Microbiology80:315–318.
Gordon, B. F., Quan, A. L. 1972. Susceptibility of chlamydia to antibacterial drugs: tests in cell culture. Antimicrobial Agents and Chemotherapy2:242–244.
Hammerschlag, M. R., Gleyzer, A. 1983. In vitro activity of a group of broad spectrum cephalosporins and other β lactam antibiotics againstChlamydia trachomatis. Antimicrobial Agents and Chemotherapy23:493–494.
Hobson, D., Lee, N., Bushell, A. C., Withana, N. 1982. The activity of β lactam antibiotics againstChlamydia trachomatis in McCoy cell cultures, pp. 249–259. In: Mårdh, P.-A. (ed.), Chlamydial infections. New York: Elsevier Biomedical.
Johnson, F. W. A. 1975. A comparison of staining techniques for demonstrating group A chlamydia in tissue culture. Medical Laboratory Technology32:233–238.
Johnson, F. W. A., Hobson, D. 1976. Factors affecting the sensitivity of replicating McCoy cells in the isolation and growth of chlamydia A (TRIC agents). Journal of Hygiene70:441–451.
Johnson, F. W. A., Hobson, D. 1977. The effect of penicillin on genital strains ofChlamydia trachomatis in tissue culture. Journal of Antimicrobial Chemotherapy3:49–56.
Johnson, P. M., Phua, K. K., Perkins, H. R., Hart, C. A., Bucknall, R. C. 1984. Antibody to streptococcal cell wall peptidoglycan polysaccharide polymers in seropositive and seronegative rheumatic disease. Clinical Experimental Immunology55:115–124.
Moulder, J. W., Norosel, D. L., Officer, J. E. 1963. Inhibition of the growth of agents of the psitticosis group byd-cycloserine and its specific reversal byd-alanine. Journal of Bacteriology85:707–711.
Newall, W. J., Jones, R. B. 1983. Disulphide linked oligomers of the major outer membrane protein of chlamydia. Journal of Bacteriology154:998–1001.
Perkins, H. R., Allison, A. C. 1963. Cell wall constitutents of Rickettsia and Psitticosis lymphogranuloma organisms. Journal of General Microbiology30:469–480.
Schachter, J., Caldwell, H. D. 1980. Chlamydiae. Annual Review of Microbiology34:285–309.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
How, S.J., Hobson, D. & Hart, C.A. Studies in vitro of the nature and synthesis of the cell wall ofChlamydia trachomatis . Current Microbiology 10, 269–274 (1984). https://doi.org/10.1007/BF01577140
Issue Date:
DOI: https://doi.org/10.1007/BF01577140