Abstract
Preparations of intact genomic DNA from 23 strains ofPropionibacterium freudenreichii were compared by digestion with restriction endonucleases and subsequent transverse alternating field electrophoresis (TAFE). Seven restriction enzymes,AsnI,DraI,HpaI,SnaBI,SpeI,SspI, andXbaI, produced DNA fragments useful for strain comparisons. A characteristic restriction fragment pattern was identified for 18 of the 23 strains. Estimates for the genome size of theP. freudenreichii strains ranged from 1.6×106 to 2.3×106 base pairs based on the sum of fragment sizes obtained with restriction digests. Restriction endonuclease patterns resolved by TAFE are useful for strain identification.
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Manuscript #6337 of the Oklahoma State Agricultural Experiment Station, Oklahoma State University, Stillwater, Oklahoma.
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Rehberger, T.G. Genome analysis ofPropionibacterium freudenreichii by pulsed-field gel electrophoresis. Current Microbiology 27, 21–25 (1993). https://doi.org/10.1007/BF01576829
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DOI: https://doi.org/10.1007/BF01576829