Abstract
An enzyme immunoassay (EIA) inhibition method was devised that detects antibodies against the M-factor, a major protein antigen in histoplasmin. Purified M-factor still contains a large amount of a carbohydrate antigen impurity, but this can be inactivated by periodate oxidation. The resulting product improves the performance of the EIA to detect anti-M by (a) giving a more specific measurement, (b) increasing the percent inhibition in sera that contain antibodies to M-factor, and (c) increasing the precision in measuring negative reactions. An inhibition of 20% or greater is recommended for recording a result as positive.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
Armitage, P. 1971. Statistical methods in medical research. New York: John Wiley and Sons.
Azuma, I., Kanetsuna, F., Tanaka, Y., Yamamura, Y., Carbonell, L. M. 1974. Chemical and immunological properties of galactomannans obtained fromHistoplasma duboisii, Histoplasma capsulatum, Paracoccidioides brasiliensis, andBlastomyces dermatitidis. Mycopathologia et Mycologia Applicata54:111–125.
Food and Drug Administration, USA 1977. Skin test antigens, proposed implementation of efficacy review. Federal Register42:52674–52687.
Green, J. H., Harrell, W. K., Gray, S. B., Johnson, J. E., Bolin, R. C., Gross, H., Bradley-Malcolm, G. 1976. H and M antigens ofHistoplasma capsulatum: preparation of antisera and location of these antigens in yeast-phase cells. Infection and Immunity14:826–831.
Heiner, D. C. 1958. Diagnosis of histoplasmosis using precipitin reactions in agar gel. Pediatrics22:616–629.
Kaufman, L. 1980. Serodiagnosis of fungal diseases, pp. 628–646. In: Lennette, E. H., Balows, A., Hausler, W. J., Jr., Truant, J. P. (eds.), Manual of clinical microbiology, 3rd ed. Washington, DC: American Society for Microbiology.
Knowles, J. B., Reiss, E., Brock, E. G., Bragg, S. L., Pine, L., Aloisio, C. H. 1983. Characterization of monoclonal antibodies to histoplasmin factors. Abstracts of the Annual Meeting of the American Society for Microbiology, p. 389.
Palmer, D. F., Kaufman, L., Kaplan, W., Cavallaro, J. J. 1977. Serodiagnosis of mycotic diseases. Springfield, IL: Charles C. Thomas.
Pine, L., Gross, H., Bradley-Malcolm, G., George, J. R., Gray, S. B., Moss, C. W. 1977. Procedures for the production and separation of H and M antigens in histoplasmin: chemical and serological properties of the isolated products. Mycopathologia61:131–141.
Reiss, E., Hutchinson, H., Pine, L., Ziegler, D. W., Kaufman, L. 1977. Solid-phase competitive binding radioim-munoassay for detecting antibody to the M antigen of histoplasmin. Journal of Clinical Microbiology6:598–604.
Sweet, G. H., Cimprich, R. S., Cook, A. C., Sweet, D. E. 1979. Antibodies in histoplasmosis detected by use of yeast and mycelial antigens in immunodiffusion and electroimmunodiffusion. American Review of Respiratory Diseases120:441–449.
Tompkins, V. N. 1965. Soluble antigenic constituents of yeast phaseHistoplasma capsulatum. American Review of Respiratory Diseases, Supplement92:126–133.
Wilson, M. B., Nakane, P. L. 1978. Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies, pp. 215–235. In: Knapp, W., Holubar, K., Wick, G. (eds.), Immunofluorescence and related staining techniques. Amsterdam: Elsevier.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Brock, E.G., Reiss, E., Pine, L. et al. Effect of periodate oxidation on the detection of antibodies against the M-antigen of histoplasmin by enzyme immunoassay (EIA) inhibition. Current Microbiology 10, 177–180 (1984). https://doi.org/10.1007/BF01576782
Issue Date:
DOI: https://doi.org/10.1007/BF01576782