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A phenotypic and genetic study of sucrose nonfermenting strains ofVibrio mimicus andVibrio cholerae

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Abstract

Sixty-six strains unable to ferment sucrose and resemblingVibrio mimicus andV. cholerae were submitted to an extensive phenotypic characterization. DNA-DNA homology among selected strains and the type strain ofV. cholerae was studied by the S1 endonuclease method. Seven sucrose-negative strains were shown to have the phenotypic properties of and a high percentage DNA relatedness toV. cholerae and a low level of homology withV. mimicus. Eight luminescent strains phenotypically most closely resembledV. mimicus; however, two of these were shown to have a high level of DNA homology withV. cholerae and a low level of relatedness toV. mimicus. A single strain was found to be phenotypically and genetically unrelated to eitherV. cholerae orV. mimicus and may represent a new species. The remaining strains were phenotypically shown to beV. mimicus, and selected strains were shown to have a high percentage DNA homology withV. mimicus and a low level of homology withV. cholerae. Problems associated with the identification of these strains and differential traits are discussed.

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Authors and Affiliations

  1. Commonwealth Institute of Health, The University of Sydney, Sydney, Australia

    Patricia M. Desmarchelier

  2. Sir George Fisher Centre for Tropical Marine Studies, James Cook University, Townsville, Queensland, Australia

    John L. Reichelt

Authors
  1. Patricia M. Desmarchelier
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  2. John L. Reichelt
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Desmarchelier, P.M., Reichelt, J.L. A phenotypic and genetic study of sucrose nonfermenting strains ofVibrio mimicus andVibrio cholerae . Current Microbiology 10, 41–47 (1984). https://doi.org/10.1007/BF01576046

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