Abstract
Using somatic cell hybrids, the gene for beta-2 microglobulin has been assigned to human chromosome 15. We found it of interest to study a number of human lymphoid cell lines in light of this finding, to analyze whether spontaneously occurring loss or reduction of beta-2 microglobulin could be correlated with any aberration in chromosome 15. The Daudi cell line was shown to be devoid of any beta-2 microglobulin in total extracts. Chromosome analysis showed that one of the two chromosomes 15 was deleted in the region q14↔q21 on the long arm; in some metaphases, both chromosomes were deleted in this region. The K562 cell line was found to express very low (if any) membrane-associated beta-2 microglobulin. Chromosome analysis showed that this line was near-triploid, with two normal chromosomes 15 and one translocation chromosome t(15;18) involving the long arm of chromosome 15, whereby the segment proximal to the breakage point in band q15 was lost. The Namalwa cell line showed a reduction in membrane-associated and total beta-2 microglobulin. Chromosome analysis showed this line to contain one chromosome 15 which was shorter than its normal homolog. The deletion could be identified as such in the region q14↔q21 in Daudi cells, but is probably somewhat smaller than the one in Daudi cells. Since analyses of beta-2 microglobulin production and chromosomes 15 on several other human cells failed to reveal any abnormalities in either of these respects, we postulate that genes responsible for beta-2 microglobulin synthesis and membrane expression could be located in the region q14→q21 on the long arm of chromosome 15. Since beta-2 microglobulin associated with the membrane was found to be absent in the K562 line, where total beta-2 microglobulin was nearly as high as in cell lines with “normal” membrane expression, it is suggested that membrane expression of beta-2 microglobulin can be regulated independently
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Abbreviations
- BSS:
-
Earle's balanced salt solution
- Q banding:
-
bands obtained by fluorescence with Quinacrine Mustard
- R banding:
-
bands obtained by fluorescence with Acridine Orange
- FITC:
-
fluorescein isothiocyanate
- EBV:
-
Epstein-Barr virus
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Zeuthen, J., Friedrich, U., Rosén, A. et al. Structural abnormalities in chromosome 15 in cell lines with reduced expression of beta-2 microglobulin. Immunogenetics 4, 567–579 (1977). https://doi.org/10.1007/BF01575690
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DOI: https://doi.org/10.1007/BF01575690