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Interspecies and interantigenic evaluation of a crossreactive rat idiotype

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Abstract

An interstrain crossreactive idiotype (ID 1), present on rat antibodies to streptococcal group A carbohydrate (SACHO), was examined for both antigen and species specificity. In a test employing a sensitive radioimmunoassay, ID 1 was undetectable in both preimmunization sera and on rat antibodies to poly-glutamic acid50-alanine50 obtained from animals which subsequently expressed ID 1 on anti-SACHO antibodies. Antigenically distinct from lambda light (L)-chain isotypic determinants, ID 1 was found to require antibody heavy-chain and L-chain interaction for complete expression. The inability to detect ID 1 on anti-SACHO antibodies produced by goats, rabbits, chickens, and nurse sharks suggests the species specificity of ID 1. These and previous observations on idiotypy favor the theory that germline genes are important in coding for rat antibody diversity to SACHO.

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Abbreviations

SACHO:

streptococcal group A carbohydrate

GASV:

group A streptococcal vaccine

RIA:

radioimmunoassay

H:

heavy

L:

light- poly-GA, poly-L-glutamic acid50-l-alanine50

λ :

lambda

κ :

kappa

R23G:

Rat 23 IgC anti-SACHO antibody

R55G:

Rat 55 IgG anti-SACHO antibody

anti-R23G:

absorbed and fractionated rabbit antiidiotypic antiserum

NaSCN:

sodium thiocyanate

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  1. Richard P. Stankus

    Present address: Department of Medicine, Clinical Immunology Section, Tulane University Medical School, New Orleans, Louisiana

Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Oregon Health Sciences Center, 97201, Portland, Oregon

    Richard P. Stankus & Gerrie A. Leslie

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  1. Richard P. Stankus
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  2. Gerrie A. Leslie
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Stankus, R.P., Leslie, G.A. Interspecies and interantigenic evaluation of a crossreactive rat idiotype. Immunogenetics 4, 233–243 (1977). https://doi.org/10.1007/BF01575663

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  • DOI: https://doi.org/10.1007/BF01575663

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