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Genetic control of IgM responses to (T,G)-A — L

H-2 andIg-1 linkage

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Abstract

The primary antibody response to aqueous immunization with a low molecular-weight lot of (T,G)-A — L (#420) was studied in congenic pairs of inbred mouse strains. Two new genetic controls were identified, both of which quantitatively regulate the production of IgM anti-(T,G)-A — L antibody. Testing of F1 and F2 progeny demonstrated that one of these genes is linked to the major histocompatibility (H-2) complex, and that high response is dominant over low response. Whether this gene is identical toIr-1A is not yet known. The other gene, designatedIg-TGAL M , is linked to the immunoglobulin heavy-chain allotype locus (Ig-1) and is expressed in a genedose dependent manner. Following secondary challenge with (T,G)-A — L 420, quantitative differences in IgG antibody response were observed inIr-1A high-responder congenics differing only at theIg-1 locus. Breeding studies, however, failed to demonstrate any linkage between this locus and the quantitative control of IgG anti-(T,G)-A — L antibody. These data demonstrate thatH-2-linked immune response genes can regulate IgM as well as IgG antibody responses, that genetic control of the IgM response to (T,G)-A — L is linked toIg-1, and that bothH-2-linked andIg-1-linked genes may simultaneously affect an IgM antibody response to the same antigen.

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Abbreviations

(T,G)-A — L:

poly-l-(Tyr,Glu)-poly-d,l-Ala-poly-l-Lys

NMS:

normal mouse serum

SRBC:

sheep red blood cells

i.p.:

intraperitoneal

PBS:

phosphate-buffered saline

RAMG:

polyvalent rabbit anti-mouse globulin

2-Me:

2-Mercaptoethanol

2-MeS:

2-Me-sensitive

PFC:

plaque-forming cells

ABC:

antigen-binding cells

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Authors and Affiliations

  1. Department of Pathology, Stanford University School of Medicine, 94305, Stanford, California

    Sean M. Smith, Dolly B. Ness, James A. Talcott & F. Carl Grumet

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  1. Sean M. Smith
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  2. Dolly B. Ness
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  3. James A. Talcott
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  4. F. Carl Grumet
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Smith, S.M., Ness, D.B., Talcott, J.A. et al. Genetic control of IgM responses to (T,G)-A — L. Immunogenetics 4, 221–232 (1977). https://doi.org/10.1007/BF01575662

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  • DOI: https://doi.org/10.1007/BF01575662

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