Abstract
Four cell lines derived from four-day-old SWR/J♀×SJL/J♂ mouse blastocysts have been assayed for their expression of H-2 specificities with pauci- and monospecific H-2 typing sera. Direct microcytotoxicity and indirect absorption studies reveal many deviations from expected expression of particular H-2 specificities based on the cell lines' genotypes and onH-2 typing of adult F1 lymphocytes. No pattern of selective expression of public or private specificities ofD-end orK- end specificities or of inclusion groups was noted. At least one public or private specificity of eachD q,K q,D s, andK s region is present, indicating that part of each product is expressed. The partial expression of H-2 specificities is discussed structurally, in terms of how incomplete H-2 molecules may be present on the cell surface, and developmentally, in terms of how the variant H-2 specificities may be involved in cell positioning during ontogenesis.
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References
Amos, D.B., Bashir, H., Boyle, W., MacQueen, M., and Tiilikainen, A.: A simple microcytotoxicity test.Transplantation 7:220–223, 1969
Bennett, D., Boyse, E.A., and Old, L.J.: Cell surface immunogenetics in the study of morphogenesis.In Silvestri, L.G. (ed.):Cell Interactions. pp. 247–263, North Holland, Amsterdam, 1971
Blankenhorn, E.P. and Douglas, T.: Location of the gene specifying theta antigen in the mouse.J. Hered. 63:259–263, 1972
Bodmer, W.F.: Evolutionary significance of theHL-A system.Nature 237:139–145, 183, 1972
Cantor, J., Shapiro, S.S., and Sherman, M.I.: Chondroitin sulfate synthesis by mouse embryonic, extra-embryonic, and teratoma cellsin vitro.Dev. Biol. 50:367–377, 1976
Ceppellini, R.: Old and new facts and speculations about transplantation antigens of man.In Amos, B. (ed.):Progress in Immunology, pp. 973–1025, Academic Press, New York, 1971
David, C.S.: Major histocompatibility system of the mouse.In Götze, D. (ed.):Organization of Major Histocompatibility Systems in Animals. Springer-Verlag, New York, in press, 1976
Edelman, G.M.: Perspective.In Kahan, B. and Reisfeld, R. (ed.):The Cell Surface, Immunological and Chemical Approaches. pp. 260–266, Plenum Press, New York, 1975
Edidin, M.: Histocompatibility genes, transplantation antigens and pregnancy.In Kahan, B.D., and Reisfeld, R. (eds.):The Transplantation Antigens. pp. 75–114, Academic Press, New York, 1972
Edidin, M.: The appearance of cell surface antigens in the development of the mouse embryo. A study of cell surface Differentiation.In McLaren, A. (ed.):Embryogenesis in Mammals: A Ciba Foundation Symposium. Elsevier, Amsterdam, in press, 1976
Gangal, S.G., Merchant, D.L., and Shreffler, D.C.: Characterization of the H-2 antigens of L-M mouse cells grown in culture.J. Natl. Cancer Inst. 36:1151–1159, 1966
Gooding, L.R. and Edidin, M.: Cell surface antigens of a mouse testicular teratoma. Identification of an antigen physically associated with H-2 antigens on tumor cells.J. Exp. Med. 140:61–78, 1974
Gooding, L.R., Hsu, Y.C., and Edidin, M.: Expression of teratoma-associated antigens on murine ova and early embryos.Dev. Biol. 49:479–486, 1976
Graziano, K.D. and Edidin, M.: Serological quantitation of histocompatibility-2 antigen and the determination of H-2 in adult and fetal organs.In Lengerová, A. and Vojtíšková, M. (eds.):Procedings of the Symposium on Immunogenetics of the H-2 System, pp. 251–256, Karger, Basel, 1971
Hauptfeld, V. and Klein, J.: Molecular relationship between private and public H-2 antigens as determined by antigen redistribution method.J. Exp. Med. 142:288–298, 1975
Klein, D., Merchant, D.J., Klein, J., and Scheffler, D.C.: Persistence of H-2 and some non-H-2 antigens on long-term cultured mouse cell lines.J. Natl. Cancer Inst. 44:1149–1160, 1970
Klein, J.:Biology of the Mouse Histocompatibility-2 Complex. pp. 313–315, Springer-Verlag, New York, 1975
Klein, J.: The ontogenetic development of H-2 antigens in vivo and in vitro.In Matoušek, J. (ed.):Blood Groups of Animals. pp. 405–414, Junk, The Hague, 1965
Pizzaro, O., Hoecker, G., Rubinstein, P., and Ramos, A.: The distribution in the tissues and development of H-2 antigens of the mouse.Proc. Natl. Acad. Sci. U.S.A. 47:1900–1906, 1961
Searle, R.F., Sellens, M.H., Elson, J., Jenkinson, E.J., and Billington, W.D.: Detection of alloantigens during preimplantation development and early trophoblast differentiation in the mouse by immunoperoxidase labeling.J. Exp. Med. 143:348–359, 1976
Sherman, M.I.: Long term culture of cells derived from mouse blastocysts.Differentiation 3:51–67, 1975a
Sherman, M.I.: The culture of cells derived from mouse blastocysts.Cell 5:343–349, 1975b
Snell, G.D.: Catalog of mouse alloantisera. Mimeographed publication of National Institutes of Health, 1968; Supplements 1969, 1973, 1974
Snell, G.D., Démant, P., and Cherry, M.: AnH-2 chart arranged to show postulated structural features ofH-2.In Lengerová, A. and Vojtíšková, M. (eds.):Proceedings of the Symposium on Immunogenetics of the H-2 System, pp. 2–9, Karger, Basel, 1971a
Snell, G.D., Démant, P., and Cherry, M.: Hemagglutination and cytotoxic studies of H-2.Transplantation 11:210–237, 1971b
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Ostrand-Rosenberg, S., Hammerberg, C., Edidin, M. et al. Expression ofHistocompatibility-2 antigens on cultured cell lines derived from mouse blastocysts. Immunogenetics 4, 127–136 (1977). https://doi.org/10.1007/BF01575652
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DOI: https://doi.org/10.1007/BF01575652