Abstract
Seven different Chinese hamster cell lines were found to vary greatly in their sensitivity to inhibitors of the mitochondrial ATPase. In plating-efficiency experiments, Chinese hamster lung V79 and bone marrow M3-1 cells were approximately 10,000-fold more resistant to oligomycin, 100-fold more resistant to efrapeptin, and 10-fold more resistant to ossamycin and leucinostatin than were ovary CHO or peritoneal B14 cells. In vitro experiments indicated that the increased resistance of V79 versus CHO cells to these inhibitors was due to an increased resistance of the mitochondrial ATPase. Heat-inactivation experiments indicated that there was a difference in the structure of the mitochondrial ATPase of V79 and CHO cells. Genetic experiments indicated that the difference in the sensitivity of V79 and CHO cells to inhibitors of the ATPase and the difference in the structure of the mitochondrial ATPase of V79 and CHO cells was due to a difference in both a nuclear and a cytoplasmic gene.
Similar content being viewed by others
Literature cited
Schatz, G., and Mason, T.L. (1974).Annu. Rev. Biochem. 43:51–87.
Borst, P., and Grivell, L.A. (1978).Cell 15:705–723.
Tzagoloff, A., Macino, G., and Sebald, W. (1979).Annu. Rev. Biochem. 48:419–441.
Bunn, C.L., Wallace, D.C., and Eisenstadt, J.M. (1974).Proc. Natl. Acad. Sci. U.S.A. 71:1681–1685.
Wallace, D.C., Bunn, C.L., and Eisenstadt, J.M. (1975).J. Cell Biol. 67:174–188.
Wallace, D.C. (1981).Mol. Cell Biol. 1:697–710.
Blanc, H., Wright, C.T., Bibb, M.J., Wallace, D.C., and Clayton, D.A. (1981).Proc. Natl. Acad. Sci. U.S.A. 78:3789–3793.
Kearsey, S.E., and Craig, I.W. (1981).Nature 290:607–608.
Doersen, C.J., and Stanbridge, E.J. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:4549–4553.
Harris, M. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:5404–5608.
Howell, N., Huang, P., Kelliher, K., and Ryan, M.L. (1983).Somat. Cell Genet. 9:143–163.
Breen, G.A.M., and Scheffler, I.E. (1980).J. Cell Biol. 86:723–729.
Lichtor, T., and Getz, G.S. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:324–328.
Lichtor, T., Tung, B., and Getz, G.S. (1979).Biochemistry 18:2582–2590.
Breen, G.A.M. (1982).Mol. Cell. Biol. 2:772–781.
Howell, N., and Sager, R. (1979).Somat. Cell Genet. 5:833–845.
Howell, N. (1983).Somat. Cell Genet. 9:1–24.
Wiseman, A., and Attardi, G. (1979).Somat. Cell Genet. 5:241–262.
Ditta, G., Soderberg, K., Landy, F., and Scheffler, I.E. (1976).Somat. Cell Genet. 2:331–344.
Soderberg, K.L., Mascarello, J.T., Breen, G.A.M., and Scheffler, I.E. (1979).Somat. Cell Genet. 5:225–240.
deFrancesco, L., Scheffler, I.E., and Bissell, M.J. (1976).J. Biol. Chem. 251:4588–4595.
Breen, G.A.M., and Scheffler, I.E. (1979).Somat. Cell Genet. 5:441–451.
Maiti, I.B., deSouza, C., and Thirion, J.-P. (1981).Somat. Cell Genet. 7:567–582.
Malczewski, R.M., and Whitfield, C.D. (1982).J. Biol. Chem. 257:8137–8142.
Soderberg, K.L., Ditta, G.S., and Scheffler, I.E. (1977).Cell 10:697–702.
Ditta, G., Soderberg, K.L., and Scheffler, I.E. (1977).Nature 268:64–67.
Burnett, K.G., and Scheffler, I.E. (1981).J. Cell Biol. 90:108–115.
Bunn, C.L., and Eisenstadt, J.M. (1977).Somat. Cell Genet. 3:611–627.
Molloy, P.L., and Eisenstadt, J.M. (1979).Somat. Cell Genet. 5:585–595.
Doersen, C.J., and Stanbridge, E.J. (1982).Mol. Cell Biol. 2:694–700.
Kuhns, M.C., and Eisenstadt, J.M. (1981).Somat. Cell Genet. 7:737–750.
Lagarde, A.E., and Siminovitch, L. (1979).Somat. Cell Genet. 5:847–871.
Webster, K.A., Oliver, N.A., and Wallace, D.C. (1982).Somat. Cell Genet. 8:223–244.
Oliver, N.A., and Wallace, D.C. (1982).Mol. Cell. Biol. 2:30–41.
Puck, T.T., Cicciura, S.M., and Robinson, A. (1958).J. Exp. Med. 108:945–955.
Worton, R.G., Ho, C.C., and Duff, C. (1977).Somat. Cell Genet. 3:27–45.
Ford, D.K., and Yerganian, G. (1958).J. Natl. Cancer Inst. 21:393–425.
Ziegler, M.L., and Davidson, R.L. (1979).J. Cell. Physiol. 98:627–635.
Yerganian, G. (1958).J. Natl. Cancer Inst. 20:705–728.
Hsu, T.C., and Humphrey, R.M. (1965).Tex. Rep. Biol. Med. 23:321–327.
Yerganian, G., and Leonard, M. (1961).Science 133:1600–1601.
Hsu, T.C., and Zenzes, M.-T. (1964).J. Natl. Cancer Inst. 32:857–869.
Hsu, T.C., and Kellogg, D.S. (1960).J. Natl. Cancer Inst. 25:221–235.
Lin, C.C., Chang, T.D., and Niewezas-Late, V. (1971).Can. J. Genet. Cytol. 13:9–13.
Veomett, G., Shay, J., Hough, P.V.C., and Prescott, D.M. (1976). InMethods in Cell Biology, Vol. 13, (ed.) Prescott, D.M. (Academic Press, New York), pp. 1–6.
Norwood, T.H., Ziegler, C.J., and Martin, G.M. (1976).Somat. Cell Genet. 2:263–270.
Gefter, M.L., Margulies, D.H., and Scharff, M.D. (1977).Somat. Cell Genet. 3:231–236.
Horstman, L.L., and Racker, E. (1970).J. Biol. Chem. 254:1336–1344.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951).J. Biol. Chem. 193:265–275.
Lardy, H., Reed, P., and Lin, C.-H.C. (1979).Fed. Proc. 34:1707–1710.
Linnett, P.E., and Beechey, R.B. (1979).Methods Enzymol. 55:472–519.
Singer, T.P. (1979).Methods Enzymol. 55:454–462.
Heytler, P.G. (1979).Methods Enzymol. 55:462–471.
Galper, J.B., and Darnell, J.E. (1971).J. Mol. Biol. 57:363–367.
Eiermann, W., Aquila, H., and Klingenberg, M. (1977).FEBS Lett. 74:209–214.
Merle, P., and Kadenbach, B. (1982).Eur. J. Biochem. 125:239–244.
Anderson, S., Bankier, A.T., Barrell, B.G., deBruijn, M.H.L., Coulson, A.R., Drouin, J., Eperon, I.C., Nierlich, D.P., Roe, B.A., Sanger, F., Schreier, P.H., Smith, A.J.H., Staden, R. and Young, I.G. (1981).Nature 290:457–465.
Bibb, M.J., Van Etten, R.A., Wright, C.T., Walberg, M.W. and Clayton, D.A. (1981).Cell 26:167–180.
Anderson, S., DeBruijn, M.H.L., Coulson, A.R., Eperon, I.C., Sanger, F., and Young, I.G. (1982).J. Mol. Biol. 156:683–717.
Macino, G., and Tzagoloff, A. (1980).Cell 20:507–517.
Sebald, W., Wachter, E., and Tzagoloff, A. (1979).Eur. J. Biochem. 100:599–607.
Colson, A.-M., Goffeau, A., Briquet, M., Weigel, P., and Mattoon, J.R. (1974).Mol. Gen. Genet. 135:309–326.
Saunders, G.W., Rank, G.H., Kustermann-Kuhn, B., and Hollenberg, C.P. (1979).Mol. Gen. Genet. 175:45–52.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Simmons, W.A., Breen, G.A.M. Biochemical genetics of the mammalian oxidative phosphorylation system: Analysis of the difference in the sensitivity of various Chinese hamster cell lines to inhibitors of the mitochondrial ATP synthase complex. Somat Cell Mol Genet 9, 549–566 (1983). https://doi.org/10.1007/BF01574258
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01574258