Summary
Experiments conducted in microcosms containing loam soil samples inoculated with eitherE. coli orPseudomonas spp. donor and recipient cells showed that bacterial cells survived and conjugated over a 24-h incubation period.E. coli transconjugants were detected 6 h after donor and recipient strains were introduced into sterile soil samples. In non-sterile soil samples, transconjugants were detected between 8 and 24 h incubation.Pseudomonas transconjugants were recovered from sterile soil samples between 6 and 12 h after their introduction and as early as 2 h in non-sterile soil. The results show that genetic interactions occur in non-sterile soil in relatively short periods of time at relatively high transfer frequencies (10−3 to 10−4). Studies on genetic interactions in soil are becoming necessary in risk assessment/environmental impact studies prior to the release of genetically engineered or modified organisms into uncontained environments.
Similar content being viewed by others
References
Anson, J.G. and G. MacKinnon. 1984. NovelPseudomonas plasmid involved in aniline degradation. Appl. Environ. Microbiol. 48: 868–869.
Bradley, D.E. 1981. Conjugative pili of plasmids inEscherichia coli K-12 andPseudomonas species. In: Molecular Biology, Pathogenicity and Ecology of Bacterial Plasmids (Levy, S.B., R.C. Clowes and E.L. Koenig, eds.), pp. 217–226, Plenum Press, New York.
Furukana, K. and A.M. Chakrabarty. 1982. Involvement of plasmids in total degradation of chlorinated biphenyls. Appl. Environ. Microbiol. 44: 619–626.
Gealt, M.A., M.D. Chai, K.B. Alpert and J.D. Boyer. 1985. Transfer of plasmids pBR322 and pBR325 in wastewater from laboratory strains ofEscherichia coli to bacteria indigenous to the waste disposal system. Appl. Environ. Microbiol. 49: 836–841.
Graham, J.B. and C.A. Istock. 1978. Gene exchange inBacillus subtilis in soil. Rapid procedure for detection and isolation of large and small plasmids. Gene exchange inBacillus subtilis in soil. Mol. Gen. Genet. 166: 287–290.
Kado, C.I. and S.T. Liu. 1981. Rapid procedure for detection and isolation of large and small plasmids. J. Bacteriol. 145: 1356–1373.
Kelly, W.J. and D.C. Reanney. 1984. Mercury resistance among soil bacteria: ecology and transferability of genes encoding resistance. Soil Biol. Biochem. 16: 1–8.
Mach, P.A. and D.J. Grimes. 1982. R-plasmid transfer in a wastewater treatment plant. Appl. Environ. Microbiol. 44: 1395–1403.
Reanney, D.C., W.P. Roberts and W.J. Kelly. 1982. Genetic interactions among microbial communities. In: Microbial interactions and communities. Vol. 1 (Bull, A.T. and J.H. Slater, eds.), pp. 287–322, Academic Press, New York.
Reanney, D.C., P.C. Gowland and J.H. Slater. 1983. Genetic interaction among microbial communities. Symp. Soc. Gen. Microbiol. 34: 379–421.
Simon, R., U. Priefer and A. Puhler. 1983. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria. Biotechnology 1: 784–791.
Stotzky, G. and V.N. Krasovsky. 1981. Ecological factors that affect the survival, establishment, growth and genetic recombination of microbes in natural habitats. In: Molecular Biology, Pathogenicity and Ecology of Bacterial Plasmids (Levy, S.B., R.C. Clowes and E.L. Koenig, eds.), pp. 31–42, Plenum Press, New York.
Trevors, J.T. 1987. Survival ofEscherichia coli donor, recipient and transconjugant cells in soil. Water Air Soil Pollut. 34: 409–414.
Trevors, J.T. and M.E. Starodub. 1987. R-plasmid transfer in non-sterile agricultural soil. System. Appl. Microbiol. 9: 312–315.
Trevors, J.T., K.M. Oddie and B.H. Belliveau. 1985. Metalresistance in bacteria. FEMS Microbiol. Rev. 32: 39–54.
van Elsas, J.D., J.T. Trevors and M.E. Starodub. 1988. Bacterial conjugation between pseudomonads in the rhizosphere of wheat. FEMS Microbiol. Ecol. 53: 299–306.
Weinberg, S.R. and G. Stotzky. 1972. Conjugation and genetic recombination ofEscherichia coli in soil. Soil Biol. Biochem. 4: 171–180.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Berg, G., Trevors, J.T. Bacterial conjugation betweenEscherichia coli andPseudomonas spp. donor and recipient cells in soil. Journal of Industrial Microbiology 5, 79–84 (1990). https://doi.org/10.1007/BF01573856
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01573856