Abstract
The results of this study indicate that the ART-1 and Ly-1 rat alloantigens are synonymous with each other and also with the leukocyte-common (L-C) antigen which has been previously identified as a major glycoprotein of rat thymocytes and T and B lymphocytes. This conclusion is supported by the following observations: (i) when labeling of rat lymphoid cells was studied with a fluorescence-activated cell sorter, the profiles obtained were similar for labeling with ART-1 and Ly-1 alloantibodies and a monoclonal antibody to L-C antigen: (ii) this labeling was almost completely inhibited by purified L-C antigen: (iii) preincubation with L-C antigen completely inhibited binding of the alloantibodies in a cellular radioimmunoassay; (iv) the cytotoxic effect of the alloantibodies was completely abolished by preincubation with purified L-C antigen; (v) the strain distribution of the ART-1 and Ly-1 alloantigens was identical for 11 rat strains and in linkage analysis the ART-1 and Ly-1 alloantigens were found to cosegregate.
Genetic linkage studies have shown that the L-C antigen locus is unlinked to the major histocompatibility antigen (RT1), the immunoglobulin light chain (1k) and to the coat color gene (C) loci.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BSA:
-
Bovine serum albumin
- DAB:
-
Dulbecco's salt solution
- FCS:
-
Fetal calf serum
- L-C antigen:
-
Leucocyte-common antigen
- LN:
-
Lymph node
- TDL:
-
Thoracic duct lymphocytes
References
Carter, P. B. and Sunderland, C. A.: Rat alloantisera ART and Ly-1 detect a polymorphism of a leucocyte-common antigen.Transplant. Proc. 11: 1646–1647, 1979
DeWitt, C. W. and McCullough, M.: Ag-F: serological and genetic identification of a new locus in the rat governing lymphocyte membrane antigens.Transplantation 19: 310–317, 1975
Fabre, J. W. and Morris, P. J.: The definition of a lymphocyte-specific alloantigen system in the rat (Ly-1).Tissue Antigens 4: 238–246, 1974
Fabre, J. W. and Williams, A. F.: Quantitative serological analysis of rabbit anti-rat lymphocyte serum and preliminary biochemical characterisation of the major antigen recognised.Transplantation 23: 349–359, 1977
Gahmberg, C. G., Häyry, P. and Andersson, L. C.: Characterisation of surface glycoproteins of mouse lymphoid cells.J. Cell Biol. 68: 642–653, 1976
Gutman, G. A. and Weissman, I. L.: Inheritance and strain distribution of a rat immunoglobulin allotype.J. Immunol. 107: 1390–1393, 1971
Howard, J. C. and Scott, D. W.: The identification of sera distinguishing marrow-derived and thymus-derived lymphocytes in the thoracic duct.Immunology 27: 903–922, 1974
Howard, J. C., Butcher, G. W., Galfrè, G., and Milstein, C.: Monoclonal anti-rat MHC (H-1) alloantibodies.Curr. Top. Microbiol. Immunol. 81: 54–60, 1978
Hunt, S. V. and Williams, A. F.: The origin of cell surface immunoglobulin of marrow-derived and thymus-derived lymphocytes of the rat.J. Exp. Med. 139: 479–496, 1974
Jensenius, J. C. and Williams, A. F.: The binding of anti-immunoglobulin antibodies to rat thymocytes and thoracic duct lymphocytes.Eur. J. Immunol. 4: 91–97, 1974
Lubaroff, D. M.: An alloantigenic marker on rat thymus and thymus-derived cells.Transplant. Proc. 5: 115–118, 1973
Lubaroff, D. M.: Antigenic markers on rat lymphocytes.Cell. Immunol. 29: 147–158, 1977
Lubaroff, D. M., Rasmussen, G. T., and Hunt, H. D.: Comparative studies of T-lymphocyte antigens in the rat: Are ART, Ly, Pta and Ag-F the same?Transplant. Proc. 11: 1642–1645, 1979
Mason, D. W.: The class of surface immunoglobulin on cells carrying IgG memory in rat thoracic duct lymph: The size of the subpopulation mediating IgG memory.J. Exp. Med. 143: 1122–1130, 1976
Mason, D. W. and Williams, A. F.: The kinetics of antibody binding to membrane antigens in solution and at the cell surface.Biochem. J. 187: 1–20, 1980
Michaelson, J., Scheid, M., and Boyse, E. A.: Biochemical features of Ly-5 alloantigen.Immunogentics 9: 193–197, 1979
Owen, R. D.: Earlier studies of blood groups in the rat.Ann. N. Y. Acad. Sci. 97: 37–42, 1962
Standring, R., McMaster, W. R., Sunderland, C. A., and Williams, A. F.: The predominant heavily glycosylated glycoproteins at the surface of rat lymphoid cells are differentiation antigens.Eur. J. Immunol. 8: 832–839, 1978
Sunderland, C. A., McMaster, W. R., and Williams, A. F.: Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes.Eur. J. Immunol. 9: 155–159, 1979
Trowbridge, I. S., Nilsen-Hamilton, M., Hamilton, R. T., and Bevan, M. J.: Preliminary characterization of two thymus-dependent xenoantigens from mouse lymphocytes.Biochem. J. 163: 211–217, 1977
Williams, A. F., Barclay, A. N., Letarte-Muirhead, M., and Morris, R. J.: Rat Thy-1 antigens from thymus and brain: Their tissue distribution, Purification and chemical composition.Cold Spring Harbor Symp. Quant. Biol. 41: 51–61, 1976
Wonigeit, K.: Characterization of the RT-Ly-1 and RT-Ly-2 alloantigenic systems by congenic rat strains.Transplant. Proc. 11: 1631–1635, 1979
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Carter, P.B., Sunderland, C.A. Biochemical identification of rat ART-1 and Ly-1 alloantigens. Immunogenetics 10, 583–593 (1980). https://doi.org/10.1007/BF01572592
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01572592