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Genetic aspects of the tolerance to allografts induced at metamorphosis in the toadXenopus laevis

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Abstract

Genetic aspects of tolerance to allografts induced at metamorphosis in the toadXenopus laevis were studied in one sibship expressing four different major histocompatibility complex (MHC) haplotypes. Tolerance by skin grafting was induced during metamorphosis in thiourea-blocked individuals, a technique that allows prolonged observation of graft behavior at this stage. Four classes of mutually tolerant animals could be determined. The use of antisera recognizing red blood cell antigens segregating with mixed lymphocyte reaction (MLR) haplotypes revealed that the four abovementioned classes corresponded to the four MHC genotypes of the family. The tolerance is, therefore, preferentially induced to antigens not dependent on the MHC. Under certain circumstances tolerance can also be induced to MHC antigens, provided that the animals differ at the level of one MHC haplotype only. Study of MLR during ontogeny suggested that, between sibs, only the two MLR haplotype differences were stimulatory at metamorphosis, whereas in larval and adult animals a one-haplotype difference was enough for stimulation.

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Authors and Affiliations

  1. Basel Institute for Immunology, Grenzacherstrasse 487, CH-4058, Basel, Switzerland

    Louis Du Pasquier

  2. Département de Biologie Animale, Université, Genève, Switzerland

    Xavier Chardonnens

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  1. Louis Du Pasquier
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  2. Xavier Chardonnens
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Du Pasquier, L., Chardonnens, X. Genetic aspects of the tolerance to allografts induced at metamorphosis in the toadXenopus laevis . Immunogenetics 2, 431–440 (1975). https://doi.org/10.1007/BF01572313

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  • DOI: https://doi.org/10.1007/BF01572313

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