Abstract
Two-way and one-way lymphocyte cultures were prepared between rabbits of four inbred strains, B, Y, C, and A, in all possible combinations. There was no reaction between B and Y, and between C and A, but significant reactions were found in the remaining combinations. Cells of B and Y appeared to be the best responders, and those of C and A strains the best stimulators. Based on the premise that the mixed lymphocyte and graft-versus-host reactions are similar, we tentatively concluded that such an asymmetrical response is due to (a) allelic differences at loci responsible for the reaction or (b) differences in homozygosity between strains. Mixed lymphocyte cultures between parental strains and the hybrid generation in a cross of B × C were also prepared. Survival times of grafts of the B backcross to B rabbits correlated slightly with the results of the mixed lymphocyte reaction.
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References
Bach, F.H. and Hirschhorn, K.: Lymphocyte interaction: a potential histocompatible test in vitro.Science 143:813–814, 1964
Bain, B. and Lowenstein, L.: Genetic studies on the mixed leukocyte reaction.Science 145:1315–1316, 1964
Chai, C.K.: Effect of inbreeding in rabbits.J. Hered. 60:64–69, 1969
Chai, C.K.: Genetic studies of histocompatibility in rabbits: Identification of major and minor genes.Immunogenetics 1:126–132, 1974
Cohen, C. and Tissot, R.G.: The effect of the RL-A locus and MLC locus on graft survival in the rabbit.Transplantation 18:150–154, 1974
Festenstein, V.P., Abbasi, K., Sachs, J.A., and Oliver, R.T.D.: Serologically undetectable immune responses in transplantation.Transplant. Proc. 4:219–225, 1972
Gasser, D.L. and Silvers, W.K.: Genetic determinants of immunological responsiveness.Adv. Immunol. 18:1–66, 1974
Lozner, E.C., Sachs, A.H., Shearer, G.M., and Terry, W.D.: B-cell alloantigens determined by theH-2 linked Ir region are associated with mixed lymphocyte culture stimulation.Science 183:757–759, 1974
Sørensen, S.F., Bildsoe, P., and Simonsen, M.: Effect of strong and weak histocompatibility antigens on the mixed lymphocyte culture reaction in rats.Acta Pathol. Microbiol. 79:475–482, 1971
Wilson, B.D. and Elkins, W.L.: Proliferative interaction of lymphocytes in vitro and in vivo. Mani-festation of immunocompetence.In W.O. Rieke (ed.):Proceedings of the Third Annual Leukocyte Culture Conference; p. 391. Appleton-Century-Crofts, New York, 1969
Yunis, E.J. and Amos, D.B.: Three closely linked genetic systems relevant to transplantation.Proc. Natl. Acad. Sci. USA 68:3031–3035, 1971
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Chai, C.K., Lerner, C.P. Asymmetrical mixed lymphocyte reaction between inbred strains of rabbit. Immunogenetics 2, 363–368 (1975). https://doi.org/10.1007/BF01572305
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DOI: https://doi.org/10.1007/BF01572305