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Degradation of 2-fluorobenzoate by a pseudomonad

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Abstract

Pseudomonas (spp), isolated from a complex petrochemical sludge, was able to utilize 2-fluorobenzoate as its sole source of carbon and energy. At the end of the growth phase, about 42% of the organically bound fluoride was released. Catechol, 3-fluorocatechol, and 6-fluorodihydrodihydroxybenzoate were confirmed as intermediates by chromatographic and spectral analyses. During 2-fluorobenzoate metabolism, fluoride is eliminated before the aromaticity of the ring is lost. Twofold higher levels of catechol 1,2-oxygenase were detected in 2-fluorobenzoate-grown cells compared with cells grown on benzoate. When used as assay substrates, 3-chlorocatechol showed less catechol 1,2-oxygenase activity than catechol or 4-chlorocatechol. The ability to degrade 4-fluorobenzoate could be transferred toPseudomonas (spp) by the conjugal transfer of plasmid pWR1 fromPseudomonas sp. B13.

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Authors and Affiliations

  1. Department of Microbiology and Biotechnology Center, Faculty of Science, M.S. University of Baroda, 390 002, Baroda, India

    Kalpit A. Vora, Cherry Singh &  Vinod V. Modi

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  1. Kalpit A. Vora
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  2. Cherry Singh
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  3. Vinod V. Modi
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Vora, K.A., Singh, C. & Modi, V.V. Degradation of 2-fluorobenzoate by a pseudomonad. Current Microbiology 17, 249–254 (1988). https://doi.org/10.1007/BF01571323

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