Abstract
An aerobic bacterial consortium was shown to degrade 2,4,6-trinitrotoluene (TNT). At an initial concentration of 100 ppm, 100% of the TNT was transformed to intermediates in 108 h. Radiolabeling studies indicated that 8% of [14C]TNT was used as biomass and 3.1% of [14C]TNT was mineralized. The first intermediates observed were 4-amino-2,6-dinitrotoluene and its isomer 2-amino-4,6-dinitrotoluene. Prolonged incubation revealed signs of ring cleavage. Succinate or another substrate—e.g., malic acid, acetate, citrate, molasses, sucrose, or glucose—must be added to the culture medium for the degradation of TNT. The bacterial consortium was composed of variousPseudomonas spp. The results suggest that the degradation of TNT is accomplished by co-metabolism and that succinate serves as the carbon and energy source for the growth of the consortium. The results also suggest that this soil bacterial consortium may be useful for the decontamination of environmental sites contaminated with TNT.
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Literature Cited
APHA (1988) Standard methods for the examination of water and waste water, 15th edn. New York: American Public Health Association
Bartha R, Pramer D (1965) Features of a flask method for measuring the persistence and biological effects of pesticides in soil. Soil Sci 100:68–70
Bechard G, Bisaillon JG, Beaudet R (1990) Degradation of phenol by a bacterial consortium under methanogenic condition. Can J Microbiol 36:573–578
Bridge JE, Swanston C, Lane RE, Davis TP (1942) Trinitrotoluene poisoning. Proc R Soc Lond [Biol] 35:553–560
Bueding E, Jolliffe N (1946) Metabolism of trinitrotoluene (TNT) in vitro. J Pharmacol Exp Ther 88:300–312
Cartwright NJ, Cain RR (1959) Bacterial degradation of the nitrobenzoic acids. Biochem J 71:248–261
Channon HJ, Mills GT, Williams RT (1944) The metabolism of 2,4,6-trinitrotoluene (TNT). Biochem J 38:70–85
Dale HH (1921) The fate of TNT in the animal body. Med Res Counc (GB) Spec Rep Ser 58:53–61
Eddy JH (1945) Methionine in the treatment of toxic hepatitis. Am J Med Sci 210:374–380
Fernando T, Bumpus JA, Aust SD (1990) Biodegradation of TNT (2,4,6-trinitrotoluene) byPhanerochaete chrysosporium. Appl Environ Microbiol 56:1666–1671
Hamilton A (1921) Trinitrotoluene as an industrial poison. J Ind Hyg 3:102–116
Harvey SD, Fellows RJ, Cataldo DC, Bean RM (1990) Analysis of 2,4,6-trinitrotoluene and its transformation products in soils and plant tissues by high performance liquid chromatography. J Chromatogr 518:361–374
Jensen HL, Larsen GL (1967) Microorganisms that decompose nitro-aromatic compounds, with special reference to dinitro-o-cresol. Acta Agric Scand 17:115–126
Kaplan DL, Kaplan AM (1982) Mutagenicity of 2,4,6-trinitrotoluene surfactant complexes. Bull Environ Contam Toxicol 28:33–38
Klausmeier RE, Osmon JL, Walls DR (1974) The effect of trinitrotoluene on microorganisms. Dev Ind Microbiol 15:309–317
Lemberg R, Callaghan JP (1944) Metabolism of symmetrical trinitrotoluene. Nature 154:768
Mans RJ, Novelli GD (1961) Measurement of the incorporation of radioactive amino acids into protein by a filter paper disk method. Arch Biochem Biophys 94:48–53
McCormick NG, Feeherry FE, Levinson HS (1976) Microbial transformation of 2,4,6-trinitrotoluene and other nitroaromatic compounds. Appl Environ Microbiol 31:949–958
Nay MW Jr., Randall CW, King PH (1974) Biological treatability of trinitrotoluene manufacturing waste water. J Water Pollut Control Fed 46:485–497
Osmon JL, Klausmeier RE (1972) The microbial degradation of explosives. Dev Ind Microbiol 14:247–252
Pearson J (1948) The reduction of nitro compounds at the dropping mercury electrode. Trans Faraday Soc 44:683–697
Pereira WE, Short DL, Manigold DB, Roscio PK (1979) Isolation and characterization of TNT and its metabolites in ground waters by gas chromatograph-mass spectrometer-computer techniques. Bull Environ Contam Toxicol 21:554–562
Sax NI (1963) Dangerous properties of industrial materials, 2nd edn. New York: Reinhold Publishing Corp.
Saz AK, Slie RB (1954) The inhibition of organic nitro reductase by aureomycin in cell free extracts. II. Cofactor requirements for the nitro reductase enzyme complex. Arch Biochem Biophys 51:5–16
Simpson JR, Evans WC (1953) The metabolism of nitrophenols by certain bacteria. Biochem J 55:24
Stanier RY, Palleroni NJ, Doudoroff M (1966) The aerobicPseudomonads: a taxonomic study. J Gen Microbiol 43:154–271
Takahaski H, Taniguchi S, Egami F (1963) Comparative biochemistry, Vol. 5. New York: Academic Press Inc.
Traxler RW, Wood E, Delaney JM (1974) Bacterial degradation of alpha-TNT. Dev Ind Microbiol 16:71–76
Voegtlin C, Hooper CW, Johnson JM (1919) Trinitrotoluene poisoning. US Public Health Rep 34:1307–1313
Westfall BB (1954) The reduction of symmetrical trinitrotoluene by a succinic dehydrogenase preparation. J Pharmacol Exp Ther 79:23–26
Won WD, Heckly RJ, Glover DJ, Hoffsommer JC (1974) Metabolic disposition of 2,4,6-trinitrotoluene. Appl Environ Microbiol 27:513–516
Won WD, Disalvo LH, Ng J (1976) Toxicity and mutagenicity of 2,4,6-trinitrotoluene and its microbial metabolites. Appl Environ Microbiol 31:576–580
Zucker M, Nason A (1955) Nitroaryl reductase fromNeurospora crassa. Methods Enzymol 2:406–411
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Boopathy, R., Manning, J., Montemagno, C. et al. Metabolism of 2,4,6-trinitrotoluene by aPseudomonas consortium under aerobic conditions. Current Microbiology 28, 131–137 (1994). https://doi.org/10.1007/BF01571053
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DOI: https://doi.org/10.1007/BF01571053