Abstract
The adherence to hamster tracheal epithelium, of mucoid and nonmucoid clinical isolates ofPseudomonas aeruginosa from cystic fibrosis patients, was studied using tracheal organ cultures. Tracheal cultures were infected with 107 colony-forming units per ml of either mucoid or nonmucoid clinical isolates ofP aeruginosa. The tracheal explants were rinsed at various time intervals to remove nonadherent bacteria, fixed, and prepared for transmission-and scanning-electron microscopy. Mucoid isolates were seen adhering to the ciliated epithelium as early as 4 h after initiation of infection, whereas nonmucoid isolates were only observed adhering at 6 to 8 h after infection. Mucoid organisms were found as clusters of bacteria embedded in an extensive extracellular matrix. The nonmucoid isolates were generally found as single organisms with no evidence of an extracellular matrix. These results suggest that the prevalence of mucoid isolates ofP. aeruginosa in cystic fibrosis may be due to adherent properties of the mucoid organism.
Similar content being viewed by others
Literature Cited
Blackwood, L. L., Penington, J. E. 1981. Influence of mucoid coating on clearance ofPseudomonas aeruginosa from lungs. Infection and Immunity32:443–448.
Chakrabarty, A. M. 1980. Practical applications of genetic engineering techniques, pp. 137–149. In: Dean, D. H., Johnson, L. F., Kimball, P. C., Perlman, P. S. (eds.), Gene structure and expression. Columbus: Ohio State University Press.
Collier, A. M., baseman, J. B. 1973. Organ culture technique withMycoplasmas. Annals of the New York Academy of Science255:277–289.
Collier, A. M., Peterson, L. P., Baseman, J. B. 1977. Pathogenesis of infection withBordetella pertussis in hamster tracheal organ culture. Journal of Infectious Diseases, Suppl.136:S196-S203.
Costerton, J. W., Brown, M. R. W., Sturgess, J. M. 1979. The cell envelope: its role in infection inPseudomonas aeruginosa, pp. 41–62. In: Doggett, R. G. (ed.), Clinical manifestations of infection and current therapy. New York: Academic Press.
Doggett, R. G., Harrison, G. M., Stillwell, R. N., Wallis, E. S. 1966. An atypicalPseudomonas aeruginosa associated with cystic fibrosis of the pancreas. Journal of Pediatrics68:215–221.
Evans, L. R., Linker, A. 1973. Production and characterization of the slime polysaccharide ofPseudomonas aeruginosa. Journal of Bacteriology116:915–924.
Fisher, M. W., Devlin, H. B., Gnabasik, F. J. 1969. New immunotype schema forPseudomonas aeruginosa based on protective antigens. Journal of Baceriology98:835–836.
Govan, J. R. W. 1975. Mucoid strains ofPseudomonas aeruginosa: The influence of culture medium on the stability of mucus production. Journal of Medical Microbiol8:513–522.
Govan, J. R. W., Fyfe, J. A. M. 1978. MucoidPseudomonas aeruginosa and cystic fibrosis: Resistance of the mucoid form to carbenicillin, flucloxacillin, and tobramycin and the isolation of mucoid variantsin vitro. Journal of Antimicrobial Chemotherapy4:233–240.
Govan, J. R. W., Fyfe, J. A. M., Lam, K., Costerton, J. W. 1982. Physiological. genetic, and rat lung studies on alginate-producing mucoidPseudomonas aeruginosa isolatedin vitro and from patient with cystic fibrosis. Journal of Infectious Diseases, in press.
Jensen, S. E., Phillippe, L., Ten Tseng, J., Stemke, G. W., Campbell, J. N., 1980. Purification and characterization of exocellular proteases produced by a clinical isolated and a laboratory strain ofPseudomonas aeruginosa. Canadian Journal of Microbiology26:77–86.
Klein, J. D., Collier, A. M. 1974. Pathogenesis of human parainfluenza type 3 virus infection in hamster tracheal organ culture. Infection and Immunity10:883–888.
Kulczycki, L. L., Murphy, T. M., Bellanti, J. A. 1978.Pseudomonas colonization in cystic fibrosis: A study of 160 patients. Journal of the American Medical Association240:30–34.
Kurioka, S., Matsuda, M. 1976. Phospholipase C assay usingp-nitrophenylphosphorlcholine together with sorbitol and its application to studying the metal and detergent requirement of the enzyme. Analytical Biochemitry75:281–289.
Lam, J., Chan, R., Lam, K., Costerton, J. W. 1980. Production of mucoid microcolonies byPseudomonas aeruginosa within infected lungs in cystic fibrosis. Infection and Immunity28:546–556.
Laraya-Cuasay, L. R., Cundy, K. R., Huang, N. N. 1976.Pseudomonas carrier rates of patients with cystic fibrosis and of members of their families. Journal of Pediatrics89:23–26.
Phillips, I. 1969. Identification ofPseudomonas aeruginosa in the clinical laboratory. Journal of Medical Microbiology2:9–16.
Schwarzmann, S., Boring, J. R., III. 1971. Antiphagocytic effect of slime from a mucoid strain ofPseudomonas aeruginosa. Infection and Immunity3:762–767.
Stinson, M. W., Hayden, C. 1979. Secretion of phospholipase C byPseudomonas aeruginosa. Infection and Immunity25:558–564.
Vasil, M. L., Kabat, D., Iglewski, B. H. 1977. Structureactivity relationships of an exotoxin ofPseudomonas aeruginosa. Infection and Immunity16:353–361.
Woods, D. E., Bass, J. A., Johanson, W. G, Jr., Straus, D. C. 1980. Role of adherence in the pathogenesis ofPseudomonas aeruginosa lung infection in cystic fibrosis patients. Infection and Immunity30:694–699.
Woods, D. E., Straus, D. C., Johanson, W. G., Jr., Berry, V. K., Bass, J. A. 1980. Role of pili in adherence ofPseudomonas aeruginosa to mammalian buccal epithelial cells. Infection and Immunity29:1146–1151.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Baker, N.R., Marcus, H. Adherence of clinical isolates ofPseudomonas aeruginosa to hamster trachael epithelium in vitro. Current Microbiology 7, 35–40 (1982). https://doi.org/10.1007/BF01570977
Issue Date:
DOI: https://doi.org/10.1007/BF01570977