Abstract
Indirect immunoferritin labeling provided evidence for the de novo synthesis of antigenic cell-surface components of the daughter cell (bud) of a freshwater isolate belonging to morphotype IV of theBlastocaulis-Planctomyces group of budding and nonprosthecately appendaged prokaryotes. The cell surfaces of the buds produced by immunoferritin-labeled mother cells remained unlabeled through development to flagellated swarmers. Double-labeling experiments verified the de novo synthesis of cell-surface antigens over the entire bud, and also showed a labeling pattern indicative of intercalation of newly formed sites on the surface of the mother cell during the later stages of its maturation.
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Tekniepe, B.L., Schmidt, J.M. & Starr, M.P. Immunoferritin labeling shows de novo synthesis of surface components in buds of a prokaryote belonging to morphotype IV of theBlastocaulis-Planctomyces group. Current Microbiology 7, 1–6 (1982). https://doi.org/10.1007/BF01570971
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DOI: https://doi.org/10.1007/BF01570971