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Transformation ofHaemophilus influenzae following electroporation with plasmid and chromosomal DNA

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Abstract

Naturally transformable species, such asHaemophilus influenzae, have evolved systems for the efficient uptake and integration of DNA from the surrounding environment. We compared this competence-dependent DNA uptake system to electroporation, which has been widely used in the past few years to introduce DNA into cells, for transformingHaemophilus influenzae. Electroporation improved transformation efficiency when noncompetent cells were used and when DNA lackingHaemophilus-specific uptake sequences was used for transformation of competent cells. An increase in plasmid-to-chromosome recombination was seen when plasmid DNA containing chromosomal inserts was introduced into competent cells by electroporation, as observed previously for natural transformation.

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Authors and Affiliations

  1. Biology Department, Brookhaven National Laboratory, T6G 2J2, Upton, New York, USA

    Jane K. Setlow

  2. Provincial Laboratory of Public Health for Northern Alberta, University of Alberta, T6G 2J2, Edmonton, Canada

    William L. Albritton

Authors
  1. Jane K. Setlow
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  2. William L. Albritton
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Setlow, J.K., Albritton, W.L. Transformation ofHaemophilus influenzae following electroporation with plasmid and chromosomal DNA. Current Microbiology 24, 97–100 (1992). https://doi.org/10.1007/BF01570904

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