Abstract
Esterases and lactate-(LDH) and malate-(MDH) dehydrogenases of 34 strains ofAlcaligenes faecalis, 16 strains ofA. denitrificans subspxylosoxydans (A. xylosoxydans), 5 strains ofA. piechaudii, and 10 strains ofA. denitrificans subspdenitrificans (A. denitrificans) were analyzed by horizontal polyacrylamide-agarose gel electrophoresis. Four types of esterases were identified inA. faecalis, 3 inA. xylosoxydans, 3 inA. piechaudii, and 14 inA. denitrificans by their spectra of hydrolytic activity towards five synthetic substrates. Four variants of MDH and no LDH variant were detected in the strains ofA. faecalis. MDH and LDH were distributed in three variants among the strains ofA. xylosoxydans. Only one variant of MDH and two variants of LDH were found inA. piechaudii strains. Four variants of MDH and one variant of LDH were identified in the strains ofA. denitrificans. A correspondence analysis of these results allowed precise identification of all the strains within these four species ofAlcaligenes.
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Bizet, C., Picard, B., Tekaia, F. et al. Electrophoretic patterns of esterases and lactate- and malate-dehydrogenases fromAlcaligenes species. Current Microbiology 27, 79–84 (1993). https://doi.org/10.1007/BF01570862
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DOI: https://doi.org/10.1007/BF01570862