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Adherence ofStaphylococcus epidermidis to human pharyngeal epithelial cells: Evidence for lipase-sensitive adhesin and glycoprotein receptor

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Abstract

The adherence of eight strains ofStaphylococcus epidermidis to human pharyngeal epithelial cells (PEC) was investigated. All bacterial strains were nonencapsulated and non-slime producers. Binding was mannose resistant and was not related to surface hydrophobicity and surface charge of bacteria. Adherence to epithelial cells was reduced four- to tenfold (P<0.01) on pretreatment of bacteria with lipase while neuraminidase, phospholipase C, trypsin, and sodium periodate did not alter their binding. The surface carbohydrate profile of bacteria was studied by monitoring adherence to Lectin-Sepharoses. The bacteria did not conform to any pattern, and there was no relation to strain variation. The pretreatment of PEC with trypsin and sodium metaperiodate produced a marked reduction in bacterial binding (three- to 25-fold, P<0.01), but neuraminidase, phospholipase C, and lipase did not have any such effect. These findings provide evidence that the receptors on the surface of PEC are glycoprotein in nature, while the bacterial adhesin is a lipase-sensitive material.

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Literature Cited

  1. Abaas S (1985) Binding of lectins toStreptococcus mitis cells. Acta Pathol Microbiol Immunol Scand Sect B 93:15–20

    Google Scholar 

  2. Andersson B, Leffler H, Magnusson G, Svanborg Eden C (1983) Molecular mechanisms of adhesion ofStreptococcus pneumoniae to human oropharyngeal epithelial cells. Scand J. Infect Dis Suppl 39:45–47

    PubMed  Google Scholar 

  3. Baddour LM, Christensen GD, Hester MG, Bisno AL (1984) Production of experimental endocarditis by coagulase-negative staphylococci: variability in species virulence. J Infect Dis 150:721–727

    PubMed  Google Scholar 

  4. Barrett SP (1985) Protein-mediated adhesion ofStaphylocococcus aureus to silicone implant polymer. J Med Microbiol 20:249–253

    PubMed  Google Scholar 

  5. Beachey EH, Simpson WA (1982) The adherence of group A streptococci to oropharyngeal cells: the lipoteichoic acid adhesin and fibronectin receptor. Infection 10:107–111

    PubMed  Google Scholar 

  6. Christensen GD, Simpson WA, Bisno AL, Beachey EH (1982) Adherence of slime-producing strains ofStaphylococcus epidermidis to smooth surfaces. Infect Immun 37:318–326

    PubMed  Google Scholar 

  7. Davis CP, Avots-Avotins AE, Fader RC (1981) Evidence of a bladder cell glycolipid receptor forEscherichia coli and the effect of neuraminic acid and colominic acid on adherence. Infect Immun 34:944–948

    PubMed  Google Scholar 

  8. Falkowski W, Edwards M, Schaeffer AJ (1986) Inhibitory effect of substituted aromatic hydrocarbons on adherence ofEscherichia coli to human epithelial cells. Infect Immun 52:863–866

    PubMed  Google Scholar 

  9. Hogt AH, Dankert J, Hulstaert CE, Feijen J (1986) Cell surface characteristics of coagulase-negative staphylococci and their adherence to fluorinated poly (ethylenepropylene). Infect Immun 51:294–301

    PubMed  Google Scholar 

  10. Jann K, Schmidt G, Blumenstock E, Vosbeck K (1981)Escherichia coli adhesion toSaccharomyces cerevisiae and mammalian cells: role of piliation and surface hydrophobicity. Infect Immun 32:484–489

    PubMed  Google Scholar 

  11. Quie PG, Belani KK (1987) Coagulase-negative staphylococcal adherence and persistence. J Infect Dis 156:543–547

    PubMed  Google Scholar 

  12. Russel PB, Kline J, Yoder MC, Polin RA (1987) Staphylococcal adherence to polyvinyl chloride and heparin-bonded polyurethane catheters is species dependent and enhanced by fibronectin. J Clin Microbiol 25:1083–1087

    PubMed  Google Scholar 

  13. Teti G, Chiofalo MS, Tomasello F, Fava C, Mastroeni P (1987) Mediation ofStaphylococcus saprophyticus adherence to uroepithelial cells by lipoteichoic acid. Infect Immun 55:839–842

    PubMed  Google Scholar 

  14. Tylewska S, Hjerten S, Wadstrom T (1979) Contribution of M protein to the hydrophobic surface properties ofStreptococcus pyogenes. FEMS Microbiol Lett 6:249–253

    Google Scholar 

  15. Tylewska S, Fischetti VA, Gibbon RJ (1988) Binding selectivity ofStreptococcus pyogenes and M-protein to epithelial cells differs from that of lipoteichoic acid. Curr Microbiol 16:209–216

    Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, Kuwait University, P.O. Box 24923, 13110, Safat, Kuwait

    Tulsi D. Chugh & Eiman A. Bishbishi

  2. Department of Biochemistry, Faculty of Medicine, Kuwait University, P.O. Box 24923, 13110, Safat, Kuwait

    Gary J. Burns

  3. Department of Cardiac Surgery, Ministry of Public Health, Kuwait

    Hani J. Shuhaiber

Authors
  1. Tulsi D. Chugh
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  2. Gary J. Burns
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  3. Hani J. Shuhaiber
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  4. Eiman A. Bishbishi
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Chugh, T.D., Burns, G.J., Shuhaiber, H.J. et al. Adherence ofStaphylococcus epidermidis to human pharyngeal epithelial cells: Evidence for lipase-sensitive adhesin and glycoprotein receptor. Current Microbiology 18, 109–112 (1989). https://doi.org/10.1007/BF01570834

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  • DOI: https://doi.org/10.1007/BF01570834

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