Abstract
A filter hybridization method employing bacterial samples and [125I]labeled chromosomal DNA as a probe was used for DNA-DNA hybridization. It was found that the hybrids had a thermal melting temperature very similar to that of duplexes formed by purified filterbound DNA. The difference in thermal denaturation midpoint between homologous and heterologous duplexes was determined for a number of strains ofAcinetobacter spp. andEnterobacter agglomerans. A comparison with the corresponding data obtained by the hydroxyapatite method showed good correlation between the two methods. The use of bacterial samples in filter hybridization omits the time-consuming DNA preparation procedure necessary for traditional DNA-DNA hybridization procedures. A simplified, two-step elution procedure is suggested for processing large numbers of strains.
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Tjernberg, I., Lindh, E. & Ursing, J. A quantitative bacterial dot method for DNA-DNA hybridization and its correlation to the hydroxyapatite method. Current Microbiology 18, 77–81 (1989). https://doi.org/10.1007/BF01570828
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DOI: https://doi.org/10.1007/BF01570828