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Ouabain-resistant mutants of Chinese hamster ovary cells are not directly affected in Na+, K+-ATPase

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Somatic Cell and Molecular Genetics

Abstract

The nature of biochemical lesions in a number of independent single-step ouabainresistant (OuaR) mutants of Chinese hamster ovary cells has been examined. The cellular uptake of86 Rb in the mutant cells was found to be resistant to inhibition by ouabain and correlated with the degree of resistance of the mutants to the drug. However, the plasma membrane Na+, K+-ATPase from all five of the OuaR mutants examined showed similar sensitivity to inhibition by ouabain as seen for the enzyme from the parental sensitive cells. These results provide evidence that, contrary to the general assumption, OuaR mutants of Chinese hamster ovary cells, which are widely used for genetic and quantitative mutagenesis studies, are not directly affected in the Na+, K+-ATPase.

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Authors and Affiliations

  1. Department of Biochemistry, McMaster University, L8N 3Z5, Hamilton, Canada

    Arvind Chopra & Radhey S. Gupta

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  1. Arvind Chopra
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  2. Radhey S. Gupta
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Chopra, A., Gupta, R.S. Ouabain-resistant mutants of Chinese hamster ovary cells are not directly affected in Na+, K+-ATPase. Somat Cell Mol Genet 12, 367–373 (1986). https://doi.org/10.1007/BF01570731

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  • DOI: https://doi.org/10.1007/BF01570731

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