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Further description of theLy-5 system

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Abstract

TheLy-5 system of alloantigens was reevaluated with the aid of an Ly-5 congenic mouse strain and an additional serological technique, the PA-SRBC rosette assay, not previously applied to Ly-5. In the PA-SRBC assay, SRBC to which PA (Staphylococcal Protein A) has been coupled react with antibodycoated cells to form rosettes.

The PA-SRBC assay was more sensitive than the cytotoxicity assay in detecting Ly-5 on all cell types tested, with the exception of thymocytes, on which Ly-5 was more efficiently demonstrable by the latter test. Thus, the use of both assays gave a more complete picture of theLy-5 system.

Evidently Ly-5 is expressed on most cells of the hematopoietic lineage, but on no other cells so far tested. The Ly-5+ cell population includes all known sets of T cells, prothymocytes, sets of B cells, cells of myeloid and monocyte-macrophage types and natural killer cells. Erythrocytes and proerythroblasts are Ly-5−, but the Ly-5 phenotype of less differentiated erythrocytic cells is uncertain. By means of radiation chimeras, made by restoring lethally irradiated mice with Ly-5 congenic bone marrow, the weakly Ly-5+ phenotype of liver and of kidney was shown to be due to immigrant circulating cells.

The Ly-5 phenotypes of tumors conform to this scheme of Ly-5 tissue representation. The Ly-5+ tumors included more than 40 leukemias tested, plasmacytomas, putative transformed equivalents of slg−: Lyb-2+ early B cells, a mastocytoma, a monocytic line, and three lines related to the macrophage. The other tumors tested -a sarcoma, a spontaneous mammary carcinoma, a teratocarcinoma and a neuroblastoma — were Ly-5−.

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Abbreviations

B6:

C57BL/6

BALB:

BALB/c

BM:

bone marrow cells

LN:

lymph node cells

PA-SRBC:

Protein A -coated sheep erythrocytes

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Authors and Affiliations

  1. Memorial Sloan-Kettering Cancer Center, 10021, New York, New York

    Margrit P. Scheid & Dennis Triglia

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  1. Margrit P. Scheid
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  2. Dennis Triglia
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Scheid, M.P., Triglia, D. Further description of theLy-5 system. Immunogenetics 9, 423–433 (1979). https://doi.org/10.1007/BF01570435

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  • DOI: https://doi.org/10.1007/BF01570435

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