Abstract
With the use of mixed-hemadsorption-hybrid-antibody (MHA-HA) test, H-Y antigen was studied on neonatal testicular cells and fractionated testicular cells from young mice (4–6 weeks old). H-Y antigen was undetectable on spermatogonia cells from neonatal testes but became fully expressed on late spermatids. Our data suggested that there was postmeiotic expression of H-Y antigen.
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Bennett, D.: The T-locus of the mouse.Cell 6:441–454, 1975
Bennett, D. and Boyse, E. A.: Sex ratio in progeny of mice inseminated with sperm treated with H-Y antiserum.Nature 246:308–309, 1973
Fellous, M. and Dausset, J.: Probable haploid expression of HLA antigens on human spermatozoan.Nature 225:191–195, 1970
Goldberg, E. H., Aoki, T., Boyse, E. A., and Bennett, D.: Detection of H-2 antigens on mouse spermatozoa by the cytotoxicity test.Nature 228:570–572, 1970
Hämmerling, U., Stackpole, C. W., and Koo, G. C.: Hybrid antibodies for labeling cell surface antigens.In H. Busch (ed.):Methods in Cancer Research IX, pp. 255–282, Academic Press, New York, 1973
Kierzenbaum, A. L. and Tres, L. L.: Structural and transcriptional features of mouse spermatid genome.J. Cell Biol. 65:258–270.
Koo, G. C., Mittl, L. R., and Boyse, E. A.: Antibody to testicular cells in the serum of normal mice.Immunogenetics 4:213–219, 1977
Koo, G. C., Stackpole, C. W., Boyse, E. A., Hämmerling, U., and Lardis, M.: Topographical location of H-Y antigen on mouse spermatozoa by immunoelectron microscopy.Proc. Natl. Acad. Sci. U.S.A. 70:1502–1505, 1973
Lee, D. P. and Dixon, R. L.: Antineoplastic drug effects on spermatogenesis studied by velocity sedimentation cell separation.Toxicol. App. Pharmacol. 23:20–41, 1972
Meistrich, M. L., Bruce, W. R., and Clermont, Y.: Cellular composition of fractions of mouse testis cells following velocity sedimentation separation.Exp. Cell Res. 79:213–227, 1973
Millette, C. F. and Bellve, A. R.: Temporal expression of membrane antigens during mouse spermatogenesis.J. Cell Biol. 74:86–97, 1977
Rugh, R.:The Mouse: its reproduction and development. Burgess Publishing Co., Minneapolis, Minnesota 1968
Tung, P. S. and Fritz, J. B.: Specific surface antigens on rat pachytene and successive classes of germinal cell.Dev. Biol. 64:297–315. 1978
Vojtíšková, M. and Pokorná, F.: Developmental expression of H-2 antigens in the spermatogenic cell series: possible bearing on haploid gene action.Folia Biol. (Praha) 18:1–9, 1972
Wachtel, S. S.: H-Y antigen: Genetics and serology.Immunol. Rev. 33:33–58, 1977
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Koo, G.C., Mittl, L.R. & Goldberg, C.L. Expression of H-Y antigen during spermatogenesis. Immunogenetics 9, 293–296 (1979). https://doi.org/10.1007/BF01570423
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DOI: https://doi.org/10.1007/BF01570423