Abstract
We have investigated alloantigen differences which stimulate lymphokine release and3H-TdR uptake in primary ‘one-way’ MLC among allogeneic mice. When mice differing at the wholeH-2 region were tested, MIF and immune IF release was observed, along with a marked3H-TdR uptake. Differences atK, D, orI-S-G regions stimulate both lymphokine release and3H-TdR uptake, though stronger immune IF and3H-TdR responses were observed with differences atI-S-G regions. On the other hand, when mice differing in their minor histocompatibility antigens, and notably at theMls locus, were tested, lymphokine release took place even in the absence of proliferation. Lastly, in MLC between mice differing at multiple minor loci, butH-2 andMls matched, MIF release only, and not immune IF and3H-TdR responses were observed in a few combinations. These findings show that T lymphocytes can recognize alloantigens by releasing lymphokines even without going through proliferation. Moreover, different levels of T-lymphocyte activation exist, depending on the kind of stimulating alloantigens present.
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Landolfo, S., Marcucci, F., Giovarelli, M. et al. Lymphokine production in mouse mixed lymphocyte reaction (MLR). Immunogenetics 9, 245–253 (1979). https://doi.org/10.1007/BF01570418
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DOI: https://doi.org/10.1007/BF01570418