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Allelic exclusion of surface IgM allotypes on spleen and bursal B cells in the chicken

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Abstract

The expression of IgM-1a and IgM-1b (Cμ) allotypes on the surface of splenic and bursal cells of B14 line chickens was examined by the triple-layer immunfluorescence technique. We have demonstrated that all surface Ig+ cells in both spleen and bursa expressed sIgM-1 which was subject to allelic exclusion in M-la/M-lb heterozygous chickens. Furthermore, a phenotypic equilibrium in the proportion of cells carrying the products of the twoM-1 alleles was observed.

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Abbreviations

Ig:

immunoglobulin

cIg:

cytoplasmic immunoglobulin

sIg:

surface immunoglobulin

RAC:

rabbit anti-chicken Ig serum

PBS:

phosphate buffered saline

NCS:

normal chicken serum

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Authors and Affiliations

  1. Department of Experimental Immunobiology, The Wellcome Research Laboratories, BR3 3BS, Beckenham, Kent, England

    Michael J. H. Ratcliffe & Juraj Ivanyi

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  1. Michael J. H. Ratcliffe
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  2. Juraj Ivanyi
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Ratcliffe, M.J.H., Ivanyi, J. Allelic exclusion of surface IgM allotypes on spleen and bursal B cells in the chicken. Immunogenetics 9, 149–156 (1979). https://doi.org/10.1007/BF01570405

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  • DOI: https://doi.org/10.1007/BF01570405

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