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Incorporation of [32P]- and [14C]-ATP intoEscherichia coli isocitrate lyase

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Abstract

InEscherichia coli, isocitrate lyase has been shown to be phosphorylated in vitro by [γ-32P]-ATP on histidine residues. This phosphorylation is believed to be necessary for activity of this enzyme. Previous work has shown that treatment of isocitrate lyase with acid phosphatase leads to a decrease in activity as well as a loss of incorporated [32P]-phosphate in a time-dependent manner. In addition to phosphorylation by [γ-32P]ATP, isocitrate lyase has been found to incorporate radioactive label from [α-32P]ATP and from [14C]ATP. This finding may indicate that more than one type of covalent modification occurs on this enzyme. Isocitrate lyase activity, inE. coli, may be regulated by posttranslational modification in several ways.

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Literature Cited

  1. Beevers H (1961) Metabolic production of sucrose from fat. Nature (London) 191:433–436

    Google Scholar 

  2. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye binding. Anal Biochem 72:248–254

    PubMed  Google Scholar 

  3. Garnak M, Reeves HC (1979) Purification and properties of phosphorylated isocitrate dehydrogenase fromEscherichia coli. J. Biol Chem 254:7915–7920

    PubMed  Google Scholar 

  4. Hagaishi O, Ueda K (1977) Poly (ADP-ribose) and ADP-ribosylation of proteins. Annu Rev Biochem 46:95–116

    PubMed  Google Scholar 

  5. Hoyt JC, Reeves HC (1988) In vivo phosphorylation of isocitrate lyase fromEscherichia coli D5H3G7. Biochem Biophys Res Commun 153:875–880

    PubMed  Google Scholar 

  6. Kunce CM, Trelease RN (1986) Heterogeneity of catalase in maturing and germinating cotton seeds. Plant Physiol 81:1134–1139

    Google Scholar 

  7. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685

    Google Scholar 

  8. Malloy PJ, Reeves HC, Speiss J (1984) Amino acid sequence of the phosphorylation site of isocitrate dehydrogenase fromEscherichia coli. Curr Microbiol 11:37–42

    Google Scholar 

  9. Robertson EF, Reeves HC (1987) Purification and characterization of isocitrate lyase fromEscherichia coli. Curr Microbiol 14:347–350

    Google Scholar 

  10. Robertson EF, Hoyt JC, Reeves HC (1987) In vitro phosphorylation ofEscherichia coli isocitrate lyase. Curr Microbiol 15:103–105

    Google Scholar 

  11. Robertson EF, Hoyt JC, Reeves HC (1988) Evidence of histidine phosphorylation in isocitrate lyase fromEscherichia coli. J Biol Chem 263:2477–2482

    PubMed  Google Scholar 

  12. Schieven G, Thorner J, Martin GS (1986) Protein-tyrosine kinase activity inSaccharomyces cerevisiae. Science 231:390–393

    PubMed  Google Scholar 

  13. Shapiro BM, Stadtman ER (1968) 5′-Adenylyl-O-tyrosine. The novel phosphodiester residue of adenylylated glutamine synthetase fromEscherichia coli. J Biol Chem 243:3769–3771

    PubMed  Google Scholar 

  14. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354

    PubMed  Google Scholar 

  15. de Verdier C-H (1953) The isolation of phosphothreonine from bovine casein. Acta Chem Scand 7:196–200

    Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, Arizona State University, 85287, Tempe, Arizona, USA

    Jeffrey C. Hoyt & Henry C. Reeves

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  1. Jeffrey C. Hoyt
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  2. Henry C. Reeves
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Hoyt, J.C., Reeves, H.C. Incorporation of [32P]- and [14C]-ATP intoEscherichia coli isocitrate lyase. Current Microbiology 18, 257–259 (1989). https://doi.org/10.1007/BF01570302

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