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The 25-kilodalton hemolytic protein affinity-purified from parasporal inclusions ofBacillus thuringiensis strain PG-14 (serotype 8a∶8b)

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Abstract

A simple method using an antibody-mediated affinity chromatography was developed for rapid and specific purification of the 25-kilodalton protein from alkali-solubilized and silkworm (Bombyx mori) larval gut juice-digested parasporal inclusions of theBacillus thuringiensis strain PG-14 (serotype 8a∶8b). Affinity-purified 25-kilodalton protein was highly hemolytic to red blood cells (RBCs) of two avian (chicken and goose) and six mammalian (horse, mouse, cow, rabbit, guinea pig, and sheep) species. The concentration of the 25-kolodalton protein required for 100% hemolysis was in the range of 2–16 μg/ml, and an apparent RBC species-dependent variation was observed in hemolytic activity of this protein. Of the RBCs tested, chicken and house RBCs were the most susceptible to hemolysis by this protein; sheep RBCs wre 4–8 times less susceptible than the others.

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Authors and Affiliations

  1. Institute of Biological Control, Faculty of Agriculture, Kyushu University, 812, Fukuoka, Japan

    Y. M. Yu,  M. Ohba & K. Aizawa

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  1. Y. M. Yu
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  2. M. Ohba
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  3. K. Aizawa
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Yu, Y.M., Ohba, M. & Aizawa, K. The 25-kilodalton hemolytic protein affinity-purified from parasporal inclusions ofBacillus thuringiensis strain PG-14 (serotype 8a∶8b). Current Microbiology 18, 243–246 (1989). https://doi.org/10.1007/BF01570299

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  • DOI: https://doi.org/10.1007/BF01570299

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