Skip to main content
Log in

Isolation and genetic analysis of resistant mutants to the benzimidazole fungicide benomyl inCoprinus cinereus

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

In total, 404 variants resistant to the antimicrotubule agent benomyl were isolated from UV-irradiated oidia of the basidiomyceteCoprinus cinereus. Part of the variants showed, in addition to benomyl resistance, heat sensitivity or heat dependence. Fifteen variants selected on the basis of different phenotypes were subjected to further analyses. All of the 15 variations were due to single-gene mutations, and the mutations comprised four groups (benA, benB, benC, andbenD) in terms of genetic linkage. Some of the 15 mutations affected nuclear migration in dikaryosis and/or fruiting processes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Literature Cited

  1. Borck K, Braymer HD (1974) The genetic analysis of resistance to benomyl inNeurospora crassa. J Gen Microbiol 85:51–56

    PubMed  Google Scholar 

  2. Burland TG, Gull K (1984) Molecular and cellular aspects of the interaction of benzimidazole fungicides with tubulin and microtubules. In: Trinci APJ, Ryley JF (eds) Mode of action of antifungal agents. Cambridge, London, New York, New Rochelle, Melbourne, Sydney: Cambridge University Press, pp 299–320

    Google Scholar 

  3. Burland TG, schedl T, Gull K, Dove WF (1984) Genetic analysis of resistance to benzimidazoles inPhysarum: differential expression of β-tubulin genes. Genetics 108:123–141

    PubMed  Google Scholar 

  4. Kamada T, Hamada Y, Takemaru T (1982) Autolysisin vitro of the stipe cell wall inCoprinus cinereus. J Gen Microbiol 128:1041–1046

    Google Scholar 

  5. Kamada T, Katsuda H, Takemaru T (1984) Temperaturesensitive mutants ofCoprinus cinereus defective in hyphal growth and stipe elongation. Curr Microbiol 11:309–312

    Google Scholar 

  6. Miles PG, Takemaru T, Kimura K (1966) Incompatibility factors in the natural population ofSchizophyllum commune. I. Analysis of the incompatibility factors present in fruit bodies collected within a small area. Bot Mag (Tokyo) 79:693–705

    Google Scholar 

  7. Morris NR (1986) The molecular genetics of microtubule proteins in fungi. Exp Mycol 10:77–82

    Google Scholar 

  8. Raper JR (1966) Genetics of sexuality in higher fungi. New York: Ronald

    Google Scholar 

  9. Roy D, Fantes PA (1982) Benomyl resistants ofSchizosaccharomyces pombe cold sensitive for mitosis. Curr Genet 6:195–201

    Google Scholar 

  10. Uno I, Ishikawa T (1971) Chemical and genetical control of induction of monokaryotic fruiting bodies inCoprinus macrohizus. Molec Gen Genet 113:228–239

    Google Scholar 

  11. Van Tuyl JM (1977) Genetics of fungal resistance to systemic fungicides. Ph.D. Thesis, University of Wageningen, The Netherlands

    Google Scholar 

  12. Yamamoto M (1980) Genetic analysis of resistant mutants to antimitotic benzimidazole compounds inSchizosaccharomyces pombe. Mol Gen Genet 180:231–234

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kamada, T., Sumiyoshi, T., Shindo, Y. et al. Isolation and genetic analysis of resistant mutants to the benzimidazole fungicide benomyl inCoprinus cinereus . Current Microbiology 18, 215–218 (1989). https://doi.org/10.1007/BF01570294

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01570294

Keywords

Navigation