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Increase of the antimicrobial susceptibility ofYersinia enterocolitica associated with the expression of the virulence plasmid

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Abstract

Virulent strains ofYersinia enterocolitica incubated in RPMI 1640 medium with 25 mM HEPES at 37°C were more susceptible to several antibiotics than their plasmid-free isogenic derivatives. The enumeration of viable bacteria in RPMI 1640 agar at 37°C to discriminate between plasmid-bearing and spontaneously derived, plasmid-free bacteria made it possible to show that the plasmid presence was associated with a fourfold decrease of minimal inhibitory concentration of ampicillin, streptomycin, gentamicin, chloramphenicol, and oxytetracycline. SinceY. enterocolitica is an intracellular pathogen and RPMI 1640 medium mimics the intracellular milieu, the plasmid-associated increase of susceptibility to antibiotics that are concentrated by animal cells may be of clinical relevance.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Pharmacy, University of Granada, 18001, Granada, Spain

    Maria Jimenez-Valera, Alfonso Ruiz-Bravo, Sergio M. Román & Alberto Ramos-Cormenzana

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  1. Maria Jimenez-Valera
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  2. Alfonso Ruiz-Bravo
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  3. Sergio M. Román
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  4. Alberto Ramos-Cormenzana
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Jimenez-Valera, M., Ruiz-Bravo, A., Román, S.M. et al. Increase of the antimicrobial susceptibility ofYersinia enterocolitica associated with the expression of the virulence plasmid. Current Microbiology 18, 211–214 (1989). https://doi.org/10.1007/BF01570293

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  • DOI: https://doi.org/10.1007/BF01570293

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