Abstract
The thermophilic mouldMalbranchea pulchella var.sulfurea TMD-8 produced extracellular xylanases in wheat straw hemicellulose as well as wheat straw. This mould utilized xylose less efficiently than glucose. Mycelial extracts contained xylose isomerase, xylose reductase, and xylitol dehydrogenase. Xylose isomerase was less thermostable than that from other microorganisms. However, xylitol dehydrogenase and xylose reductase were relatively more thermostable in comparison with these enzymes from other microorganisms. The affinity of xylose isomerase for xylose was very high (Km 10mM), while that of xylose reductase was low (Km 23.5mM). The xylitol dehydrogenase exhibited relatively high affinity for xylitol (Km 0.02mM). The activity of this enzyme, however, declined steeply, in the alkaline range. This is the first report on the occurrence of three intracellular enzymes, xylose isomerase, xylose reductase, and xylitol dehydrogenase in a thermophilic mould, which play an important role in xylose metabolism.
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Literature Cited
Anand L, Krishnamurthy S, Vithayathil PJ (1990) Purification and properties of xylanase from the thermophilic fungusHumicola lanuginosa (Griffon & Maublanc) Bunce. Arch Biochem Biophys 276:546–553
Barnett JA (1968) Biochemical differentiation of taxa with special reference to the yeasts. In: Ainsworth GC, Sussman AS (eds) The fungi, vol. 3. New York: Academic Press, pp 557–595
Birken S, Pisano MA (1976) Purification and properties of a polyol dehydrogenase fromCephalosporium chrysogenum. J Bacteriol 125:225–232
Chakravorty M, Vieger LA, Bacila M, Horecker BL (1962) Pentose metabolism inCandida. J Biol Chem 237:1014–1020
Chen WP, Anderson AW (1980) Extraction of hemicellulose from ryegrass straw for the production of glucose isomerase and use of the resulting straw residue for feed. Biotechnol Bioeng 22:519–531
Chiang CL, Knight SG (1960) Metabolism ofD-xylose by moulds. Nature 188:79–81
David J, Wiesmeyer H (1970) Regulation of ribose metabolism inEscherichia coli. I. The ribose catabolic pathway. Biochim Biophys Acta 208:45–50
Dubey AK, Johri BN (1987) Xylanolytic activity of thermophilicSporotrichum sp. andMyceliophthora thermophilum. Proc Indian Acad Sci (Plant Sci) 97:247–255
Emerson R (1941) An experimental study of the life cycles and taxonomy ofAllomyces. Lloydia 4:77–144
Gomes J, Gomes I, Kreiner W, Esterbauer H, Sinner M, Steiner W (1993) Production of high level of cellulase-free and thermostable xylanase by a wild strain ofThermomyces lanuginosus using beechwood xylan. J Biotechnol 30:283–297
Hasija SK, Miller CE (1971) Nutrition ofChytriomyces and its influence on morphology. Am J Bot 59:939–944
Höfer M, Betz A, Kotyk A (1971) Metabolism of the obligatory yeastRhodotorula gracilis. IV. Induction of an enzyme necessary forD-xylose catabolism. Biochim Biophys Acta 252:1–12
Horecker BL (1984) Monosaccharides and derivatives. In: Bergmeyer HV (ed) Methods of enzymatic analysis, vol. 6. Basel: Verlag Chemie, pp 465–473
Johri BN, Satyanarayana T (1986) Thermophilic moulds: perspectives in basic and applied research. Indian Rev Life Sci 6:75–100
Johri BN, Jain S, Chauhan S (1985) Enzymes from thermophilic fungi: proteases and lipases. Proc Indian Acad Sci (Plant Sci) 94:175–196
Kise S, Koizumi N, Maeda H (1988) Properties of NAD(P)H-linked aldose reductase fromCryptococcus lactativorus. J Ferment Technol 66:615–623
Kwon HJ, Kitada M, Horikoshi K (1987) Purification and properties ofD-xylose isomerase from alkalophilicBacillus No. KX-6. Agric Biol Chem 51:1983–1989
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with folin-phenol reagent. J Biol Chem 193:265–275
Maheshwari R, Kamalam PT (1985) Isolation and culture of a thermophilic fungusMelanocarpus albomyces and factors influencing the production and activity of xylanase. J Gen Microbiol 131:3017–3027
Matsuo M, Yasui T (1985) Properties of xylanase ofMalbranchea pulchella var.sulfurea no. 48. Agric Biol Chem 49:839–841
Reese ET (1946) Aerobic decomposition of cellulose by microorganisms at temperatures above 40°C. Ph.D. thesis, Pennsylvania State College, 100 pp
Rizzi M, Harwart K, Thanh NAB, Dellweg H (1989) A kinetic study of the NAD+-xylitol dehydrogenase from the yeastPichia stipitis. J Ferment Bioeng 67:25–30
Satyanarayana T, Johri BN (1983) Variation in xylanolytic activity of thermophilic fungi. Bionature 3:39–41
Satyanarayana T, Jain S, Johri BN (1988) Cellulases and xylanases of thermophilic moulds. In: Agnihotri VP, Sarbhoy AK, Kumar D (eds) Perspectives in mycology and plant pathology. New Delhi: Malhotra Publishing House, pp 24–60
Satyanarayana T, Johri BN, Klein J (1992) Biotechnological potential of thermophilic fungi. In: Arora DK, Elander RP, Mukherji KG (eds) Handbook of applied mycology, vol. 4. New York: Marcel Dekker Inc, pp 729–761
Sharma HS, Johri BN (1992) The role of thermophilic fungi in agriculture. In: Arora DK, Elander RP, Mukherji KG (eds) Handbook of applied mycology, vol. 4. New York: Marcel Dekker Inc, pp 707–728
Singh A, Schügerl K (1992) Induction and regulation ofD-xylose catabolising enzymes inFusarium oxysporum. Biochem Int 28:481–488
Smiley KL, Bolen PL (1982) Demonstration ofD-xylose reductase andD-xylitol dehydrogenase inPachysolen tannophilus. Biotechnol Lett 4:607–610
Suakane M, Tamura M, Tomimura C (1978) Physico-chemical and enzymatic properties of purified glucose isomerase fromStreptomyces olivochromogenes andBacillus stearothermophilus. Agric Biol Chem 42:909–917
Subrahmanyam A (1980) Studies onThermoascus aurantiacus Mehei. Acta Mycol 26:121–131
Suihko ML, Suomalainen I, Enari TM (1983)D-Xylose catabolism inFusarium oxysporum. Biotechnol Lett 5:525–530
Suzuki T, Onishi H (1975) Purification and properties of polyol: NADP oxidoreductase fromPichia quercuum. Agric Biol Chem 39:2389–2397
Verduyn C, Jzn JF, Dijken JP, Scheffers WA (1985) Multiple forms of xylose reductase inPachysolen tannophilus CBS 4044. FEMS Microbiol Lett 30:313–317
Wu JF, Lastick SM, Updegraff DM (1986) Ethanol production from sugars derived from plant biomass by a novel fungus. Nature 321:887–888
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Banerjee, S., Archana, A. & Satyanarayana, T. Xylose metabolism in a thermophilic mouldMalbranchea pulchella var.sulfurea TMD-8. Current Microbiology 29, 349–352 (1994). https://doi.org/10.1007/BF01570228
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DOI: https://doi.org/10.1007/BF01570228